p53-regulated transcriptional program associated with genotoxic stress-induced apoptosis.
AuthorsKho PS, Wang Z, Zhuang L, Li Y, Chew JL, Ng HH, Liu ET, Yu Q,
JournalJ Biol Chem
PubMed ID15016801
'By using a genome-wide approach, we sought the identification of p53-regulated genes involved in cellular apoptosis. To this end, we assessed the transcriptional response of HCT116 colorectal cancer cells during apoptosis induced by the anticancer drug 5-fluorouracil as the function of p53 status, and we identified 230 potential targets that ... More
Substrate degradation by the anaphase promoting complex occurs during mitotic slippage.
AuthorsLee J, Kim JA, Margolis RL, Fotedar R,
JournalCell Cycle
PubMed ID20436289
Microtubule targeting drugs are successful in chemotherapy because they indefinitely activate the spindle assembly checkpoint. The spindle assembly checkpoint monitors proper attachment of all kinetochores to microtubules and tension between the kinetochores of sister chromatids to prevent premature anaphase entry. To this end, the activated spindle assembly checkpoint suppresses the ... More
Overexpressed pituitary tumor-transforming gene causes aneuploidy in live human cells.
AuthorsYu R, Lu W, Chen J, McCabe CJ, Melmed S,
JournalEndocrinology
PubMed ID12960092
The mammalian securin, pituitary tumor-transforming gene (PTTG), is overexpressed in several tumors and transforms cells in vitro and in vivo. To test the hypothesis that PTTG overexpression causes aneuploidy, enhanced green fluorescent protein (EGFP)-tagged PTTG (PTTG-EGFP) was expressed in human H1299 cancer cells (with undetectable endogenous PTTG expression) and mitosis ... More