This is the new, improved version of SuperSignal West Pico Chemiluminescent Substrate (Cat. No. 34080, etc.).
Thermo Scientific™

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Catalog number:  34580

Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:
Sensitivity: pictogram to femtogram sensitivity
Stability: 8-hr working solution stability; 1-year kit stability at room temperature
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 6 to 24 hours
Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

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SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

For Research Use Only. Not for use in diagnostic procedures.


Product Line: SuperSignal™
Recommended Antibody Concentrations: 2°: 1:20-100K (10-50ng/mL), 1°: 1:1K (0.2- 1µg/mL)
Shipping Condition: Room Temperature
Signal Duration: 6-24 hr
Substrate Properties: Chemical Substrate
Detection Method: Chemiluminescent
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Sensitivity: Low picogram to high femtogram

Contents & storage

Sufficient for 60 mini-gel blots; 5,000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 250 mL
• SuperSignal West Pico PLUS Stable Peroxide, 250 mL

Store at room temperature.