This is the new, improved version of SuperSignal West Pico Chemiluminescent Substrate (Cat. No. 34080, etc.).
Thermo Scientific™

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Catalog number:  34580

 Related applications:

Protein Assays and Analysis

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Description

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions making it an ideal choice for most western blotting applications.

Features of SuperSignal West Pico PLUS Chemiluminescent Substrate:

Picogram to femtogram sensitivity—detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane
High signal stability—incubated blots provide stable signal duration over the critical 4 hour time period with up to 24 hours of light output under optimal conditions
Stable reagent—8-hour working solution stability; 1-year kit stability at room temperature
Economical—optimized for dilute antibody concentrations:
     --0.2 to 1.0 µg/mL primary antibody (1:1000 to 1:5000 dilution from 1 mg/mL stock)
     --10 to 50 ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1 mg/mL stock)
Exceptional robustness—provides high performance outside of the recommended antibody dilution ranges, including the most common 1:5K to 1:10K secondary antibody dilutions of a 1 mg/mL stock solution

Specifications

Detection Method: Chemiluminescent
Product Line: SuperSignal™
Product Size: 500 mL
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Shipping Condition: Room Temperature

Contents & storage

Sufficient for 60 mini-gel blots; 5,000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 250 mL
• SuperSignal West Pico PLUS Stable Peroxide, 250 mL

Store at room temperature.

Documents

Manuals & protocols