SuperSignal™ West Pico PLUS Chemiluminescent Substrate, 4 x 500 mL - FAQs

How do I make the working solution for the SuperSignal West Pico PLUS Chemiluminescent Substrate?

To prepare the working solution for the SuperSignal West Pico PLUS Chemiluminescent Substrate, follow these steps:

- Mix equal parts of the SuperSignal West Pico PLUS Luminol/Enhancer Solution and SuperSignal West Pico PLUS Stable Peroxide Solution. For example, for a mini blot, mix 5 mL of SuperSignal West Pico PLUS Luminol/Enhancer Solution with 5 mL of SuperSignal West Pico PLUS Stable Peroxide Solution.
- Ensure that the volume is sufficient to completely wet the blot and prevent it from drying out. The recommended volume is 0.1 mL/cm2.
- Note that the working solution is stable for up to 8 hours at room temperature. Avoid exposure to the sun or other intense light. Short-term exposure to lab lighting is okay.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.