Thermo Scientific™

Pierce™ 10X Western Blot Transfer Buffer, Methanol-free

Catalog number:  35040

 Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific Pierce 10X Western Blot Transfer Buffer, Methanol-free is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.

Features of 10X Western Blot Transfer Buffer, Methanol-free:

Transfer Buffer—diluted 10-fold in water, the solution is ready to use for electrophoresis (i.e., wet tank transfer from mini gels)
Easy to use—no packets to open, no powder to dissolve, and no methanol required
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—10X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 10X Western Blot Transfer Buffer is ready to use upon dilution to 1X with water; it does not contain methanol and the addition of methanol is not necessary for use in electrophoresis.

• Wet (tank) western blot transfer of proteins to a membrane
For Research Use Only. Not for use in diagnostic procedures.


Gel Type: SDS-PAGE Gel (any)
Buffer Type: Transfer Buffers
Concentrated: 10 X
Gel Compatibility: SDS-PAGE Gels
Product Line: Pierce™
Product Size: 5 L

Contents & storage

Storage: Upon receipt store at 4°C, protected from moisture. Product is shipped at ambient temperature.


Manuals & protocols