Pierce™ Fast Western Blot Kit, ECL Substrate, 1 L Kit - FAQs

View additional product information for Pierce™ Fast Western Blot Kit, ECL Substrate - FAQs (35055, 35050)

2 product FAQs found

Does my primary antibody need to be conjugated for using with the Pierce Fast Western Blot Kit, ECL Substrate (Cat. No. 35050, 35055)?

No, your primary antibody does not need to be conjugated for using with the Pierce Fast Western Blot Kit, ECL Substrate (cat. No. 35050, 35055). The kit includes all necessary reagents except the primary antibody, and it works with both mouse and rabbit primary antibodies. The HRP reagent directly binds to both mouse and rabbit primary antibodies, thus, a secondary antibody is not needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

For western blotting with the Pierce Fast Western Blot Kit, ECL Substrate (Cat. No. 35050), do I need a secondary antibody?

The kit contains the horseradish peroxidase (HRP) reagent and ECL detection reagent, therefore the kit works with blots probed with mouse or rabbit primary antibodies. The HRP reagent directly binds to both mouse or rabbit primary antibodies, thus, a secondary antibody is not needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.