The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate provides reagents and protocol for reliable and rapid, 1-hour chemiluminescent Western blotting at femtogram levels of sensitivity.
Features of this kit:
• Fast—femtogram levels of detection in about 1 hour • Convenient—no expensive hardware or vacuum required; no clogging issues • Simple—optimized protocol makes Western blot analysis easier than ever • Economical—no expensive consumables or extra equipment • Excellent stability—kit is stable for 1 year stored at 4°C • Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody
The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classical Western blotting with SuperSignal West Dura Chemiluminescent Substrate. Like other Western blotting methods, the included SuperSignal West Dura Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse primary antibody.
Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate, Mouse
Contents & storage
Sufficient For: 10 mini blots probed with mouse primary antibodies • Fast Western Antibody Diluent, 200 mL • Fast Western 10X Wash Buffer, 100 mL • Fast Western Mouse Optimized HRP Reagent, Dura, 10 mL • SuperSignal West Dura Luminol/Enhancer Solution, 50 mL • SuperSignal West Stable Peroxide Solution, 50 mL