Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Mouse

Catalog number: 35075

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate provides reagents and protocol for reliable and rapid, 1-hour chemiluminescent Western blotting at femtogram levels of sensitivity.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classical Western blotting with SuperSignal West Dura Chemiluminescent Substrate. Like other Western blotting methods, the included SuperSignal West Dura Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse primary antibody.

Related Products
Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Rabbit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Substrate: Luminol
Reactivity: Mouse
Product Size: 80 mL
Detection Method: Western Blot

Contents & storage

Sufficient For: 2 mini blots probed with mouse primary antibodies
• Fast Western Antibody Diluent, 40 mL
• Fast Western 10X Wash Buffer, 20 mL
• Fast Western Mouse Optimized HRP Reagent, Dura, 2 mL
• SuperSignal West Dura Luminol/Enhancer Solution, 10 mL
• SuperSignal West Stable Peroxide Solution, 10 mL

Store kit at 4°C.

Documents

Manuals & protocols