Dynabeads® M-280 Streptavidin, for OEM and industrial use only

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Description

Uniform and superparamagnetic Dynabeads®, 2.8 µm in diameter, with a monolayer of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The beads allow for efficient capture and downstream handling of your biotinylated ligand or target molecule. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results.

Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated antibodies, nucleic acids or other biotinylated ligands and targets. The liquid-phase behavior in combination with the superparamagnetic properties of the beads provide rapid reaction kinetics both in the coating process, separation and during washing of the analyte.

The product holds reputable Dynal high standards with respect to reproducibility and automation ability, and drives reliability for your assays.

Benefits and Features:

• Direct and fast capture of any biotinylated ligand or target
• Flexible solid-phase protocols with superior liquid-phase reaction kinetics
• Easy and reproducible handling in manufacturing
• Low charged and neutral beads, optimal for binding of proteins, peptides and antibodies
• Fast and efficient washing procedures
• High batch-to-batch reproducibility, securing consistent results in your application
• Well suited for automated protocols

Applications:
Efficient capture of biotinylated molecules. For direct⁄indirect isolation and downstream handling of nucleic acids, proteins⁄peptides and other target molecules. Due to their uniformity, excellent batch reproducibility and ease of adaptation to automated processes, Dynabeads® have become the solid phase of choice for immunoassay development.

Binding capacity:
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® M-280 Streptavidin typically binds 650-900 pmoles free biotin, 200 pmol biotinylated peptides, up to10 µg biotinylated antibody, approx. 10 µg ds-DNA or 200 pmol ss-Oligonucleotides.

Additional Info:
This specific product format is for large volume customers, available on an OEM basis. The product is also available in smaller volumes for end-users (Cat. no 112-05D, 112-06D and 602-10).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Ligand Type: Streptavidin
Form: Beads in Suspension
Product Size: 10 mL
Bead Diameter: 2.8 µm
Concentration: 10 mg⁄ml
High Throughput Compatibility: High Throughput-Compatible
Shipping Condition: Room Temperature

Contents & storage

Store at: 2-8°C

Documents

Manuals & protocols