Thermo Scientific™

Pierce™ Peroxidase IHC Detection Kit

Catalog number:  36000

 Related applications:

Cellular Imaging

| Protein Electrophoresis & Western Blotting

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The Thermo Scientific Pierce Peroxidase IHC Detection Kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counterstain and preserve experimental results with frozen or paraffin tissue sections.

Features of the Peroxidase IHC Detection Kit:

Incredibly sensitive—fifty times more sensitive than the traditional DAB (3,3'-diaminobenzidine) method and 30 times more sensitive than other metal-intensified versions of DAB
Low background, high intensity, large dynamic range—gives a crisp dark brown-black precipitate that's more intense than the dull brown precipitate observed when using DAB without enhancement; substrate delivers increased intensity with almost nonexistent background
Easy-to-use, two-component system—mix the two liquid components and the working solution is ready-to-use; sufficient substrate and other staining reagents to treat up to 500 slides
Six-hour working solution stability—unlike other DAB substrates that must be used immediately, the working solution is stable for more than six hours at room temperature
Complete kit—saves time in reagent ordering, gathering and validating reagents for the IHC application; saves money compared to purchasing of all components separately

This immunohistochemical staining kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counter-stain and preserve experimental results with frozen or paraffin tissue section that have been probed with peroxidase enzyme conjugates. The Pierce Peroxidase IHC Detection Kit eliminates the need to procure numerous reagents from various sources, resulting in a time- and cost-effective way to perform peroxidase-based tissue staining. Harris Hematoxylin nuclear stain and mounting medium allow counter-staining and tissue preservation, respectively. The detection method is compatible with direct or avidin-biotin-complex (ABC) probing strategies.

• Tissue immunohistochemistry procedures designed for DAB precipitating substrate
• DAB staining of fixed paraffin sections, smears and frozen section preparations
• Immunohisto- or immunocyto-staining using the metal-enhanced DAB substrate
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Chromogenic, Colorimetric
Product Line: Pierce™
Product Size: 1 L
Substrate: DAB (Enhanced)
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Technique: IHC (Immunohistochemistry), Immunodetection

Contents & storage

• DAB/Metal Concentrate (1X), 25 mL, store at -20°C (this solution will not freeze at -20°C)
• Universal Blocker™ Blocking Buffer in TBS, 25 mL, store at 4°C
• Peroxidase Suppressor, 2 × 1 mL, store at 4°C
• Stable Peroxide Buffer, 25 mL, store at 4°C
• BupH™ Tris Buffered Saline, 4 packs (each pack makes 5 mL), store at room temperature
• Surfact-Amps® 2 (1% Tween®-2 Detergent), 1 mL, store at 4°C
• Harris Modified Hematoxylin (without acetic acid, mercury-free), 1 mL, store at room temperature
• Mounting Medium, 6 mL, store at room temperature


Manuals & protocols