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Citations & References (3)
Thermo Scientific™
Pierce™ Peroxidase IHC Detection Kit
The Thermo Scientific Pierce Peroxidase IHC Detection Kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary componentsRead more
| Catalog Number | Quantity |
|---|---|
| 36000 | 1 L |
Catalog number 36000
Price (CLP)
-
Quantity:
1 L
The Thermo Scientific Pierce Peroxidase IHC Detection Kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counterstain and preserve experimental results with frozen or paraffin tissue sections.
Features of the Peroxidase IHC Detection Kit:
• Incredibly sensitive—fifty times more sensitive than the traditional DAB (3,3'-diaminobenzidine) method and 30 times more sensitive than other metal-intensified versions of DAB
• Low background, high intensity, large dynamic range—gives a crisp dark brown-black precipitate that's more intense than the dull brown precipitate observed when using DAB without enhancement; substrate delivers increased intensity with almost nonexistent background
• Easy-to-use, two-component system—mix the two liquid components and the working solution is ready-to-use; sufficient substrate and other staining reagents to treat up to 500 slides
• Six-hour working solution stability—unlike other DAB substrates that must be used immediately, the working solution is stable for more than six hours at room temperature
• Complete kit—saves time in reagent ordering, gathering and validating reagents for the IHC application; saves money compared to purchasing of all components separately
This immunohistochemical staining kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counter-stain and preserve experimental results with frozen or paraffin tissue section that have been probed with peroxidase enzyme conjugates. The Pierce Peroxidase IHC Detection Kit eliminates the need to procure numerous reagents from various sources, resulting in a time- and cost-effective way to perform peroxidase-based tissue staining. Harris Hematoxylin nuclear stain and mounting medium allow counter-staining and tissue preservation, respectively. The detection method is compatible with direct or avidin-biotin-complex (ABC) probing strategies.
Applications:
• Tissue immunohistochemistry procedures designed for DAB precipitating substrate
• DAB staining of fixed paraffin sections, smears and frozen section preparations
• Immunohisto- or immunocyto-staining using the metal-enhanced DAB substrate
Features of the Peroxidase IHC Detection Kit:
• Incredibly sensitive—fifty times more sensitive than the traditional DAB (3,3'-diaminobenzidine) method and 30 times more sensitive than other metal-intensified versions of DAB
• Low background, high intensity, large dynamic range—gives a crisp dark brown-black precipitate that's more intense than the dull brown precipitate observed when using DAB without enhancement; substrate delivers increased intensity with almost nonexistent background
• Easy-to-use, two-component system—mix the two liquid components and the working solution is ready-to-use; sufficient substrate and other staining reagents to treat up to 500 slides
• Six-hour working solution stability—unlike other DAB substrates that must be used immediately, the working solution is stable for more than six hours at room temperature
• Complete kit—saves time in reagent ordering, gathering and validating reagents for the IHC application; saves money compared to purchasing of all components separately
This immunohistochemical staining kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counter-stain and preserve experimental results with frozen or paraffin tissue section that have been probed with peroxidase enzyme conjugates. The Pierce Peroxidase IHC Detection Kit eliminates the need to procure numerous reagents from various sources, resulting in a time- and cost-effective way to perform peroxidase-based tissue staining. Harris Hematoxylin nuclear stain and mounting medium allow counter-staining and tissue preservation, respectively. The detection method is compatible with direct or avidin-biotin-complex (ABC) probing strategies.
Applications:
• Tissue immunohistochemistry procedures designed for DAB precipitating substrate
• DAB staining of fixed paraffin sections, smears and frozen section preparations
• Immunohisto- or immunocyto-staining using the metal-enhanced DAB substrate
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodChromogenic, Colorimetric
For Use With (Application)Immunodetection, IHC (Immunohistochemistry)
Product TypePeroxidase IHC Detection Kit
Quantity1 L
SubstrateDAB (Enhanced)
Substrate TypeHRP (Horseradish Peroxidase) Substrate
FormLiquid
Product LinePierce
Unit SizeEach
Contents & Storage
- DAB/Metal Concentrate (10X), 25 mL, store at -20°C (this solution will not freeze at -20°C)
- Universal Blocker Blocking Buffer in TBS, 250 mL, store at 4°C
- Peroxidase Suppressor, 2 × 100 mL, store at 4°C
- Stable Peroxide Buffer, 250 mL, store at 4°C
- BupH Tris Buffered Saline, 4 packs (each pack makes 500 mL), store at room temperature
- Surfact-Amps 2 (1% Tween-2 Detergent), 10 mL, store at 4°C
- Harris Modified Hematoxylin (without acetic acid, mercury-free), 100 mL, store at room temperature
- Mounting Medium, 120 mL, store at room temperature
Citations & References (3)
Citations & References
Abstract
Neutral sphingomyelinase inhibitor scyphostatin prevents and ceramide mimics mechanotransduction in vascular endothelium.
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:15142848
Recently, we showed that neutral sphingomyelinase (N-SMase) is concentrated at the endothelial cell surface in caveolae and is activated to produce ceramide in an acute and transient manner by increase in flow rate and pressure in rat lung vasculature (Czarny M, Liu J, Oh P, and Schnitzer JE, J Biol
The conserved metalloprotease invadolysin localizes to the surface of lipid droplets.
Journal:J Cell Sci
PubMed ID:19706689
'Invadolysin is a metalloprotease conserved in many different organisms, previously shown to be essential in Drosophila with roles in cell division and cell migration. The gene seems to be ubiquitously expressed and four distinct splice variants have been identified in human cells but not in most other species examined. Immunofluorescent
Identification of a structural determinant necessary for the localization and function of estrogen receptor alpha at the plasma membrane.
Journal:Mol Cell Biol
PubMed ID:12588983
'Estrogen receptors (ER) have been localized to the cell plasma membrane (PM), where signal transduction mediates some estradiol (E2) actions. However, the precise structural features of ER that result in membrane localization have not been determined. We obtained a partial tryptic peptide/mass spectrometry analysis of membrane mouse ERalpha protein. Based