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Gibco™

McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement

Catalog number: 36600021
Have Questions?
Gibco™

McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement

Catalog number: 36600021
Have Questions?
Catalog Number
36600021
Unit Size
500 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
36600021500 mL
-
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McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.


This McCoy's 5A is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• GlutaMAX™• HEPES
• Bacto-peptone 
• Phenol Red 


The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.

Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Culture Environment
CO2
Cell Type
Mammalian Cells
Classification
Animal Origin-free
Concentration
1 X
Culture Type
Mammalian Cell Culture
Form
Liquid
Product Type
McCoy's 5A Medium
Shelf Life
12 Months
Sterility
Sterile-filtered
With Additives
High Glucose, GlutaMAX, Phenol Red, Bacto-peptone, Sodium Bicarbonate
Without Additives
No HEPES, No Sodium Pyruvate
Green Features
Sustainable packaging
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Osmolality
280 - 320 mOsm/kg
pH
6.9 to 7.1
Product Line
Gibco™, GlutaMAX™
Quantity
500 mL
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

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