McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 36600021

Gibco™  Related applications: Mammalian Cell Culture

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Description

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:

WithWithout
• High Glucose• Sodium Pyruvate
• GlutaMAX™• HEPES
• Bacto-peptone
• Phenol Red

The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® McCoy's 5A is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco® McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco® McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Classification: Animal Origin-Free
Concentrated: 1 X
Culture Environment: CO2
Form: Liquid
Glutamine: GlutaMAX™-I
HEPES Buffer: No HEPES
Osmolality: 280 - 320 mOsm/kg
Phenol Red Indicator: Phenol Red
Product Size: 500 mL
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: No Sodium Pyruvate
Supplementation Required: Serum
Volume (Metric): 500 ml
pH Range: 6.9 - 7.1
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

Documents

Manuals & protocols

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