Dynabeads® SILANE Genomic DNA Kit

Catalog number: 37012D

Invitrogen™  Related applications: DNA Extraction | Diagnostic Development

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Description

For highly predictable and consistent extraction and isolation of genomic DNA from human whole blood, following a simple separation protocol. Isolates linear DNA yields relative to the number of white blood cells (WBC) present in the sample. The 1 µm Dynabeads® have an optimized silica-like surface chemistry and a high specific surface area, providing efficient kinetics and a high capacity.

Their uniform size and surface area ensure highly reproducible results and an improved performance compared to magnetic particles from alternative suppliers. Other particles often have a random size range distribution, surface area and binding capacity. This could compromise the reproducibility of your results. Dynabeads® hold reputable Dynal high standards with respect to within- and between-lot reproducibility and automation ability.

Advantages:

• Highly predictable binding per mg of beads
• Linear range of DNA yield relative to WBC-count
• High integrity and purity of isolated DNA
• Isolates 10 µg DNA from a 350 µl blood sample using 2 mg (50 µl) Dynabeads®
• Quicker than spin-columns
• More cost-effective, yet performs to the same level as spin-columns
• Automation-friendly (slow sedimentation rate + high magnetic mobility)

Applications
• Isolation of genomic DNA
• From cells and fresh/frozen blood samples
• From blood with a wide range of anticoagulants
• Well suited for automated assays
• Separate kit available for the isolation of viral DNA/RNA (Cat. no. 370-11D)

Additional Info:
Validation and customization (bead, buffer, protocol or format) can be made available on a custom OEM basis. For further information, please contact us at: ivd@invitrogen.com
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Blood
Isolation Technology: Magnetic Bead
Downstream Application: PCR, Cloning, Sequencing, Southern Blotting
High Throughput Compatibility: High Throughput-Compatible
Product Size: 96 preps
Shipping Condition: Room Temperature

Contents & storage

The kit contains: Dynabeads® MyOne™ SILANE (5 ml, 40 mg/ml, see Cat. no. 370-02D for further characteristics), binding/washing/elution buffers and a protocol optimized for isolation of genomic DNA. The kit content is sufficient for 96 isolations.
Store at: 2-8°C.

Documents

Manuals & protocols

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