Thermo Scientific™

SuperSignal™ West Dura Extended Duration Substrate

Catalog number: 37071
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Thermo Scientific™

SuperSignal™ West Dura Extended Duration Substrate

Catalog number: 37071
Have Questions?
Catalog Number
37071
Unit Size
20 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
100 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
4 x 100 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
200 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
4 x 200 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
3707120 mL
-
34075100 mL
-
34075X44 x 100 mL
-
34076200 mL
-
34076X44 x 200 mL
-
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Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate characteristics include:
Sensitivity: mid-femtogram
Stability: 24-hr working solution stability; 1-year kit stability at room temperature
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 24 hours
Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

Compare and view all western blot chemiluminescent substrates ›

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, allowing multiple film or camera exposures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Substrate
Detection Method
Chemiluminescent
Form
Liquid
For Use With (Application)
Western Blot
Membrane Compatibility
Nitrocellulose, PVDF
Product Line
SuperSignal™
Quantity
20 mL
Substrate
Horseradish Peroxidase
Recommended Antibody Concentrations
2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL)
Sensitivity
Mid-femtogram
Signal Duration
24 hr.
Sufficient For
2 Mini-Gel Blots, 200 sq. cm of Membrane
Kit Contents
Stable Peroxide Buffer, 10mL, Luminol/Enhancer, 10mL

Contents & Storage

Sufficient For: 2 mini-gel blots; 200 cm2 of membrane
• Luminol/Enhancer, 10 mL
• Stable Peroxide Buffer, 10 mL

Store at room temperature.

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