Applied Biosystems™

3730 DNA Analyzer

Catalog number:  3730S

Related applications:

Sanger Sequencing

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The 48-capillary 3730 DNA Analyzer is the Gold Standard in medium-to-high throughput genetic analysis. Use the 3730 DNA Analyzer for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample. This is upgradeable to 96 capillaries.

• Higher optical sensitivity and advanced polymers enable you to obtain higher-quality data at a lower cost.
• Multiple automation features decrease costly human errors.
• Optimized polymers increase your productivity without compromising your results.
• Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation.

Expand Your Laboratory's Possibilities
By dramatically improving your data quality, significantly reducing your cost per sample, and enabling you to perform more runs per day, the fully automated 48-capillary 3730 system gives your laboratory a tremendous technological and financial advantage. Which means you can pursue an exciting diversity of projects (and you can always upgrade to 96 capillaries when you need to).

Streamline Your Workflow
The 3730 DNA Analyzer system is engineered for highly reliable, unattended operation of up to 48 hours (applies to modules with run times longer than 30 minutes). Automation features which minimize the need for operator intervention and decrease the risk of human error include an integrated plate stacker, internal bar code reader, and onboard polymer delivery system.

Increase Productivity
The high signal-to-noise ratio ensures you get high-quality data – even when you use low-concentration samples and reagents. Labor-saving automation features minimize hands-on time and enable you to analyze more data more efficiently.

Get the Highest-Quality Sequencing and Genotyping Data
The enhanced optical design provides a higher signal-to-noise ratio and a more uniform signal profile across the array. This design, combined with our new, advanced polymer, enables the longest read lengths of any available system, and provides enhanced color balance for streamlined genotyping sample handling. In addition, exceptional sensitivity enables higher success rates across a wider range of sample templates and concentrations than other currently available systems.

One Instrument, Multiple Applications
With the 3730 DNA Analyzer, you get the highest-quality data from a wide array of applications. In addition to being ideal for high-throughput sequencing, the analyzer's optimized application assays, instrument, and analysis software also provide a complete solution for genotyping and resequencing.

Integrated Data Analysis Tools Reduce Time-to-Results
The 3730 DNA Analyzer software suite allows you to generate more meaningful data with less work. This system's labor-saving software suite includes:
• Data Collection (supplied with the instrument): Manages your instrument setup, controls instrument operations, allows real-time data visualization, and performs diagnostics. New features include: Auto-analysis with GeneMapper® and SeqScape®
• Tools to assist with regulatory and compliance requirements (In the United States, this assists with FDA 21CFR part 11).
Flexibility to use any choice in dye set option.
• Additional optimized sequencing run modules covering more applications.
• Sequencing Analysis – Designed to base-call; assign quality values; trim, display, edit and print DNA sequencing data using the KB basecaller
• Seqscape® – Provides everything you need to perform resequencing applications such as VariantSEQr™ Resequencing System
• GeneMapper® – Enables configurable, automated allele calling – a plus for high-throughput genotyping and includes tools for SNPlex™ data analysis

The instrument comes with a one year limited warranty on parts and labor.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Capacity: 16 sample plates (96-well or 384-well)
Capillary Length: 36 cm or 50 cm
Duration: 1 Year Limited Warranty on Parts and Labor
External Computer: Computer and Flat Panel Monitor
Frequency: 50⁄60 Hz
High-throughput Compatibility: High-throughput Compatible
Memory: 1 GB  400MHz RAM
Number of Capillaries: 48 Capillaries
Operating System: Microsoft Windows 7
Optics: In-capillary detection by dual-side illumination, Backside-thinned CCD for maximum optical sensitivity, Multiple Filter Sets: Z, E, G5, G5-RCT, Any4Dyeset, Any5Dyeset and S (optional)
Performance: XLRseq: 50cm - 900 QV20 base/360 min, RapidSeq: 36cm - 550 QV20 bases/36 min, FastSeq: 50cm - 700 QV20 base/60 min, GeneMapper™ Software: 36cm - up to 500 bp res w/ 0.15 bp precision in 34 min, StdSeq: 36cm - 700 QV20 base/60 min, LongSeq: 50cm - 850 QV20 base/120 min
Plate Handling: Plate Seal: Automatic on-board piercing (septa or polypropylene heat seal), Internal bar code reader, Integrated auto sampler and plate stacker
Polymer: POP-7, POP Conformational Analysis Polymer
Polymer Consumption: 36 cm: 100µl⁄run, 50 cm: 125µl⁄run
Read Length: 400 - 900 bases (varies depending on run module)
Reagent Handling: On-board reagents for up to 48 hrs unattended operation (with certain run modules), Simultaneous injection of 48 samples, Automated polymer replenishment pump for increased throughput
Sample Volume: 10-50 µL for 96-well plate, 5-30 µL for 384-well plate
System Upgrade: Upgradeable to 96 capillaries (3730xl)
Temperature Range (Metric): Temperature-controlled oven (18-70°C range) and detection cell heater
Throughput: StdSeq: 1152 samples/day (775 Kbases), XLRseq: 384 samples/day (345 Kbases), GeneMapper™ Software: 2112 samples/day (46,000 genotypes), LongSeq: 576 samples/day (490 Kbases), RapidSeq: 1920 samples/day (1050 Kbases), FastSeq: 1152 samples/day (800 Kbases)
For Use With (Equipment): 3730 DNA Analyzer
Format: 384-well plate, 96-well plate
Power Requirements: Voltage: 200-220 V or 230-240 V ±10%, Power Dissipated: About 2000 watts, Current: 15 amps max. Requires 30 amp receptacle w/in 3 m (10 ft) of instrument back.
Software Type: Data Collection Software
Starting Material: DNA
Dimensions (L x W x H) Exterior: 100 cm (W) x 73 cm (D) x 89 cm (H)
Weight: 180 kg (computer - 30 kg)
Operating Environment: Humidity: 20%-80% (non-condensing), Temperature: 18°C-30°C, Humidity: 20 to 80% (non-condensing)

Contents & storage

This system includes a 48 capillary instrument, a new Windows computer, data collection software, choice of one analysis software package, and chemistry kit.