Thermo Scientific™

SEA BLOCK Blocking Buffer

Catalog number:  37527

 Related applications:

Protein Assays and Analysis

| Protein Electrophoresis & Western Blotting

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Description

SEA BLOCK Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC, fluorescent western blotting, and other detection methods involving mammalian samples.

Compare and view all available blocking buffers

Features of SEA BLOCK Blocking Buffer:
Non-mammalian—fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
Filtered and stabilized in PBS for compatibility with most assay systems
Easy to use—can be used as supplied or diluted up to 10-fold as needed

Fish serum has several advantages over typical blocking buffers. Salmon is phylogenetically distant from mammals, which are the source of antibodies and samples used in most experiments, and its serum proteins are less likely to have specific binding interactions with proteins used in typical cell biology experiments. It is effective in stabilizing solutions and samples for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. When normal serum is causing too much background in immunohistochemistry (IHC), try this product.

SEA BLOCK Blocking Buffer is particularly effective in applications involving fluorescence imaging. It has been used to decrease background and increase signal-to-noise ratios in fluorescent western blotting and in various cellular imaging protocols.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Product Size: 500 mL
Reagent Type: Blocking Reagent
Validated Application: ELISA, Western Blot

Contents & storage

Upon receipt store at 4°C.

Documents

Manuals & protocols