Thermo Scientific™

Fish Serum Blocking Buffer

Catalog number: 37527
Thermo Scientific™

Fish Serum Blocking Buffer

Catalog number: 37527
Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples. Fish Serum Blocking Buffer is effective at stabilizing solutions and samples used for antibody-binding interactions, while at the same time minimizing the possibility of cross-reaction with mammalian sample components.
 
Catalog Number
37527
Unit Size
500 mL
Description
Fish Serum Blocking Buffer, 500 mL
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionFish Serum Blocking Buffer, 500 mL
Product TypeFish Serum Blocking Buffer
Quantity500 mL
FormLiquid
For Use With (Application)ELISA, Western Blot, Immunohistochemistry (IHC)
Concentration1X
BufferPBS
Blocking AgentFish Serum
Unit Size500 mL
Catalog Number
Unit Size
3 x 500 mL
Description
Fish Serum Blocking Buffer, 3 x 500 mL
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionFish Serum Blocking Buffer, 3 x 500 mL
Product TypeFish Serum Blocking Buffer
Quantity3 x 500 mL
FormLiquid
For Use With (Application)ELISA, Western Blot, Immunohistochemistry (IHC)
Concentration1X
BufferPBS
Blocking AgentFish Serum
Unit Size3 x 500 mL
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
37527Full specifications
500 mLFish Serum Blocking Buffer, 500 mL
-
DescriptionFish Serum Blocking Buffer, 500 mL
Product TypeFish Serum Blocking Buffer
Quantity500 mL
FormLiquid
For Use With (Application)ELISA, Western Blot, Immunohistochemistry (IHC)
Concentration1X
BufferPBS
Blocking AgentFish Serum
Unit Size500 mL
37527X3Full specifications
3 x 500 mLFish Serum Blocking Buffer, 3 x 500 mL
-
DescriptionFish Serum Blocking Buffer, 3 x 500 mL
Product TypeFish Serum Blocking Buffer
Quantity3 x 500 mL
FormLiquid
For Use With (Application)ELISA, Western Blot, Immunohistochemistry (IHC)
Concentration1X
BufferPBS
Blocking AgentFish Serum
Unit Size3 x 500 mL
Showing 2 of 2
Features of Fish Serum Blocking Buffer include:
Non-mammalian—fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
Convenient—filtered and stabilized in PBS for compatibility with most assay systems
Easy to use—can be used as supplied or diluted up to 10-fold as needed
Flexible - may be used for many different applications, including as a diluent for antibodies

Fish serum offers several advantages over typical blocking buffers. Because salmon is phylogenetically distant from mammals, which are the source of the antibodies and samples used in most experiments, its serum proteins are less likely to have specific binding interactions with proteins used in the experiments. Fish Serum Blocking Buffer is effective at stabilizing solutions and samples used for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. Try it when use of more-standard sera results in background staining in IHC. In addition, this blocker is effective in various cellular imaging protocols based on visible and near-infrared fluorescence detection. It has been used to decrease background and increase signal-to-noise ratios with near-IR fluorescent probes.

For Research Use Only. Not for use in diagnostic procedures.
  1. Alegria-Schaffer, A., et al. (2009). Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol. 463:573-99.
  2. Hypolite, J.A., et al. (2001). Am. J. Physiol. Cell. Physiol. 280:C254-64.
  3. Wang, L, et al. (2002). J. Clin. Invest. 110:1175-1184.

Contents & Storage

Upon receipt store at 4°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text