Thermo Scientific™

StartingBlock™ T20 (PBS) Blocking Buffer

Catalog number:  37539

 Related applications:

Protein Assays and Analysis

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Thermo Scientific StartingBlock Blocking Buffers are PBS and TBS formulations of a proprietary protein that provides exceptional performance and broad compatibility with membrane (Western blot) and 96-well plate (ELISA) assays.

Features of StartingBlock T20 (PBS) Blocking Buffer:

Compatible with many detection systems—Western blot, ELISA and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
Strip and reprobe without reblocking—blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Part No. 21059)
High signal:noise ratio—biotin- and serum protein-free formulation contributes to achieving signal-to-noise ratios from 10:1 to 20:1
Convenient formats—available ready-to-use 1X formulations in PBS and TBS, with and without Tween™ 20 detergent

StartingBlock Blocking Buffers performs well with an extremely wide range of antibodies, antibody combinations, other protein probing and assay systems.

Related Products
StartingBlock™ (PBS) Blocking Buffer
StartingBlock™ (TBS) Blocking Buffer
StartingBlock™ T20 (TBS) Blocking Buffer
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Product Line: StartingBlock™
Product Size: 1 L
Reagent Type: Blocking Reagent
Validated Application: ELISA, Western Blot

Contents & storage

Upon receipt store at 4°C.


Manuals & protocols