Thermo Scientific™

Guardian™ Peroxidase Conjugate Stabilizer/Diluent

Catalog number: 37548
Thermo Scientific™

Guardian™ Peroxidase Conjugate Stabilizer/Diluent

Catalog number: 37548
Catalog Number
37548
Unit Size
200 mL
Price (USD)
139.00
Your Price:
Availability
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Quantity
Catalog Number
Unit Size
1 L
Price (USD)
408.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
37548200 mL
139.00
Your Price:
***
375521 L
408.00
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Thermo Scientific Guardian Peroxidase Conjugate Stabilizer/Diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long-term storage.

Features of Guardian Peroxidase Conjugate Stabilizer/Diluent:

Preserves HRP activity—no significant loss of HRP activity over a six-month period at room temperature (1:1000 dilution) or 12 months at 4°C
Convenient—store ready-to-use dilutions (1:1000 to 1:100,000) that maintain enzyme activity in the refrigerator—no aliquoting or freezing
Assay compatible—simply add your favorite blocking buffer to create the ideal diluent for your HRP-based ELISA system or store the HRP conjugate as a 1:1000 stock solution for Western blots and dilute in the final assay buffer
Saves money—less expensive than competitor formulations or ordering new HRP conjugates

With Guardian Peroxidase Conjugate Stabilizer/Diluent, typical 1 mg/mL antibody or streptavidin peroxidase conjugates can be diluted as much as 100,000-fold for storage at 4°C. Most ELISA and blotting applications for HRP conjugates require dilution of typical 1 mg/mL stocks by at least 1000-fold (to 1 µg/mL). Sensitive assay systems, such as with chemiluminescent substrates, often require dilution up to 100,000-fold (10 ng/mL). The Guardian Solution enables these 1X working concentrations of HRP conjugate to be prepared in advanced and stored at 4°C for 12 months or at room temperature for six months.For blotting applications, it is usually best to prepare a concentration for storage that can be diluted 10-fold in assay buffer (which may contain blocking components) for final use.

Typical colorimetric ELISA procedures requires that 10 mL of HRP-conjugated secondary antibody be diluted 1000- to 5000-fold from a 1 mg/mL stock solution. That necessitates pipetting 2 to 10 µL of stock into a tube with 10 mL of diluent. More sensitive assay systems, such as Western blots with Thermo Scientific SuperSignal West Pico, Dura or Femto Chemiluminescent Substrates for HRP, require a second round of dilution to achieve the needed working concentration of HRP conjugate. With Guardian Stabilizer-Diluent, HRP conjugates can be pre-diluted and stored at concentrations that are either ready to use or easily diluted 10- to 100-fold for final use. Preparing larger volumes of pre-diluted stocks ensures consistency and greater accuracy for assays performed repeatedly day after day.
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Specifications

Quantity
200 mL
Reagent Type
Diluent, Stabilizing Reagent
Format
Liquid
Product Line
Guardian™

Contents & Storage

Upon receipt store at room temperature or 4°C.

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