1-Step™ ABTS Substrate Solution

Catalog number: 37615

Thermo Scientific™  Related applications: Protein Assays and Analysis

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ABTS Substrate is a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods.

ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase. The green product has two major absorbance peaks, 410nm and 650nm. ABTS is less sensitive than OPD and TMB in ELISA applications. It is less readily oxidized, and its color development is slower (approximately 20 minutes). This may be advantageous if unacceptable background results from the use of the OPD or TMB substrates due to higher sensitivities.

Related Products
ABTS Substrate Tablets
For Research Use Only. Not for use in diagnostic procedures.


Product Size: 250 mL
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Detection Method: Colorimetric
Substrate Properties: Chemical Substrate

Contents & storage

Store at 4°C


Manuals & protocols

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