Reactivos de la plantilla de ADN TaqMan™
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Applied Biosystems™

Reactivos de la plantilla de ADN TaqMan™

The TaqMan™ DNA Template Reagents provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleicMás información
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Número de catálogoCantidad
4019701 kit
Número de catálogo 401970
Precio (MXN)
-
Cantidad:
1 kit
The TaqMan™ DNA Template Reagents provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells. TaqMan™ beta-actin Template Reagents can be used with TaqMan™ PCR Core Reagents or SYBR™ Green PCR Core Reagents for polymerase chain reaction (PCR).

• Pre-designed primers and TaqMan™ probe eliminate assay design.
• Rapid assay development guidelines can minimize optimization time.
• Separate components provide flexibility in assay set-up.

Comprehensive Guidelines Included
We have developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalization Built-In
All our Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

For Research Use Only. Not for use in diagnostics procedures.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Modificación del cebador 3'None
Modificación del cebador 5'None
Molde controlGenomic DNA from human cells
FormatoTubo(s)
Modificación de sonda internaTAMRA Quencher (3'), FAM (5')
Línea de productosTaqMan
Tipo de productoDNA Template Reagent
Cantidad1 kit
Condiciones de envíoDry Ice
EspecieHumano
Objetivoβ-actina
Método de detecciónSonda de cebado
GC-Rich PCR PerformanceLow
Etiqueta o tinteFAM
Método de PCRqPCR
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
This kit includes: Human DNA standard curve dilution series, unknown DNA control, beta-actin Probe (FAM™ Probe), beta-actin Forward Primer, and beta-actin Reverse Primer. Store kit at -20°C.