TaqMan® DNA Template Reagents

Catalog number: 401970

Applied Biosystems™  Related applications: Real Time PCR (qPCR)

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The TaqMan® DNA Template Reagents provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells. TaqMan® beta-actin Template Reagents can be used with TaqMan® PCR Core Reagents or SYBR® Green PCR Core Reagents for polymerase chain reaction (PCR).

• Pre-designed primers and TaqMan® probe eliminate assay design.
• Rapid assay development guidelines can minimize optimization time.
• Separate components provide flexibility in assay set-up.

Comprehensive Guidelines Included
We have developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalization Built-In
All our Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


3' Primer Modification: None
5' Primer Modification: None
Control Template: Genomic DNA from human cells
Detection Method: Primer-Probe Detection
Format: Tube(s)
Includes: Control Primer-Probe Set(s) & Template(s)
Internal Probe Modification: FAM (5'), TAMRA Quencher (3')
Label or Dye: FAM™
Product Line: TaqMan™
Product Size: 1 kit
Species: Human
Target Gene: β-actin
Shipping Condition: Dry Ice

Contents & storage

This kit includes: Human DNA standard curve dilution series, unknown DNA control, beta-actin Probe (FAM™ Probe), beta-actin Forward Primer, and beta-actin Reverse Primer. Store kit at -20°C.


Manuals & protocols