eBioscience™ Phalloidin eFluor™ 570
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Invitrogen™

eBioscience™ Phalloidin eFluor™ 570

La faloidina es una toxina péptida aislada del hongo «Amanita phalloides». La faloidina se une de forma selectiva a laMás información
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Número de catálogoCantidad
41-6559-05300 pruebas
Número de catálogo 41-6559-05
Precio (USD)
-
Cantidad:
300 pruebas
La faloidina es una toxina péptida aislada del hongo «Amanita phalloides». La faloidina se une de forma selectiva a la Actina F y funciona para estabilizar los polímeros de actina al inhibir la disociación de los monómeros de actina de los extremos del filamento. Debido a que los polímeros de Actina F componen el citoesqueleto de casi todas las células eucariotas, los conjugados de faloidina pueden ser sondas útiles para visualizar la estructura de células vivas o fijas mediante microscopía. Phalloidin eFluor™ 570 se puede utilizar para etiquetar células de múltiples especies.

Phalloidin eFluor™ 570 se suministra como un líquido congelado. Para preparar la solución madre 1000X, descongele el vial, añada 30 ul de DMSO anhidro de alta calidad, mezcle y centrifugue brevemente. La solución resultante (solución madre de faloidina DMSO) debe dividirse en partes iguales, protegerse de la luz y la humedad, y almacenarse a - 20 °C. Para preparar una solución de trabajo 1X del conjugado de faloidina, añada 1 ul de solución madre de faloidina DMSO 1000X a 1 ml de PBS que contenga un 1 % de BSA

Emisión
570 nm

Excitación
555 nm

Aplicación declarada
Inmunohistoquímica, tinción inmunohistoquímica, microscopía

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Tipo de coloranteeFluor 570
Intervalo de longitud de onda de excitación555/570
Nº de pruebas300 Tests
Línea de productoseBioscience
Cantidad300 pruebas
Tipo de etiquetaeFluor
Tipo de productoFaloidina
SubCellular LocalizationCitoesqueleto
Unit SizeEach

Preguntas frecuentes

Can I combine Click-iT or Click-iT Plus reactions with phalloidin conjugates used for actin staining?

We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.

For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.

Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.