Invitrogen™

eBioscience™ Phalloidin eFluor™ 570

Catalog number:  41-6559-05

 Related applications:

Flow Cytometry

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Description

Phalloidin is a peptide toxin isolated from the Amanita phalloides mushroom. Phalloidin selectively binds F-actin and functions to stablilize actin polymers by inhibiting the dissociation of actin monomers from the filament ends. Because F-actin polymers compose the cytoskeleton of almost all eukaryotic cells, phalloidin conjugates can be useful probes for visualizing the structure of live or fixed cells using microscopy. Phalloidin eFluor™ 570 can be used to label cells of multiple species.

Phalloidin eFluor™ 570 is supplied as a frozen liquid. To prepare the 1000X stock solution, thaw the vial, add 30 uL high-quality anhydrous DMSO, mix and briefly spin down. The resulting solution (phalloidin DMSO stock) should be aliquoted, protected from light and moisture, and stored at -20°C. To prepare a 1X working solution of phalloidin conjugate, add 1 uL of 1000X phalloidin DMSO stock solution to 1 mL of PBS containing 1% BSA.

Emit
570 nm

Excite
555 nm

Reported Application
Immunocytochemistry, Immunohistochemical Staining, Microscopy

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: eFluor 570
Sub-Cellular Localization: Cytoskeleton
Excitation⁄Emission (nm): 555/570
Product Line: eBioscience™
Product Size: 300 tests

Documents

Manuals & protocols