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Ham's F-10 Nutrient Mix, GlutaMAX™ Supplement This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  41550021

Related applications:

Mammalian Cell Culture

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Description

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

This F-10 is modified as follows:

WithWithout
• GlutaMAX™• HEPES
• Phenol Red

The complete formulation is available.

Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).

cGMP Manufacturing and Quality System
Gibco® F-10 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco® F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Classification: Animal Origin-Free
Concentrated: 1 X
Culture Environment: CO2
Form: Liquid
Glutamine: GlutaMAX™-I
Green Features: Sustainable packaging
HEPES Buffer: No HEPES
Osmolality: 275 - 315 mOsm/kg
Phenol Red Indicator: Phenol Red
Product Line: Gibco®,GlutaMAX™
Product Size: 500 mL
Shipping Condition: Room Temperature
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: Sodium Pyruvate
Supplementation Required: Serum
Volume (Metric): 500 ml
pH Range: 7.0 - 7.4

Contents & storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.