Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.
This F-10 is modified as follows:
|• GlutaMAX™||• HEPES|
The complete formulation
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).cGMP Manufacturing and Quality System
Gibco™ F-10 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10% Fetal Bovine Serum
(FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2
environment to maintain physiological pH.