This product will be discontinued on December 30, 2016. For assistance, please contact your local Human Identification representative or HID Tech Support. Read discontinuation notice »

AmpFLSTR® Profiler Plus® PCR Amplification Kit

Catalog number: 4303326

Applied Biosystems™  Related applications: Forensics

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We have combined the advantages of PCR-based testing and fluorescence detection into a highly discriminating kit designed for human identification applications. The AmpFLSTR® Profiler Plus® PCR Amplification Kit amplifies nine tetranucleotide short tandem repeat (STR) loci and the Amelogenin locus in a single reaction tube. The STR loci amplified are D3S1358, D5S818, D7S820, D8S1179, D13S317, D18S51, D21S11, FGA, and vWA.

Key Features of the AmpFLSTR® Profiler Plus® PCR Amplification Kit:

• Nine tetranucleotide STR loci and the Amelogenin locus co-amplified in a single tube for ease of use
• Use in conjunction with COfiler PCR Amplification Kit
• Includes AmpliTaq Gold® DNA Polymerase for increased sensitivity and specificity
• Conveys certain rights to perform research and forensic testing services when used with an authorized thermal cycler
• Conveys the PCR service rights for paternity testing

Clear Differentiation
The Amelogenin locus is used for gender identification because products of different lengths are generated from the X and Y chromosomes.

Quality Results
The AmpFLSTR® Profiler Plus® reagents and protocols produce the quality results necessary for human identification applications. To increase the success of obtaining results in the presence of enzyme inhibitors, bovine serum albumin (BSA) has been included in the AmpFLSTR® PCR Reaction Mix.

Thermal cycler times and temperatures selectively amplify DNA sequences while providing the necessary degree of sensitivity. The recommended range of input DNA is 1.0 to 2.5 ng. Furthermore, instrument detection protocols yield reproducible results with excellent resolution and precision in sizing alleles.

AmpFLSTR® Profiler Plus® kit is subjected to rigorous quality control testing to ensure reliable performance. Experiments are performed based upon the TWGDAM recommended guidelines (Technical Working Group on DNA Analysis Methods. 1995. Guidelines for a Quality Assurance Program for DNA Analysis. Crime Lab Digest 22:21-43). These results have been summarized and submitted for peer review publication.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”
For Forensic or Paternity Use Only.


Technique: STR (Short Tandem Repeat) Analysis
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: NED™, FAM™, ROX™, JOE™
Product Size: 100 reactions
Amplicon Size: <350 bp
Sample Volume: 50 µl⁄reaction
Reaction Format: SuperMix or Master Mix
Sample Type (Specific): DNA (Genomic), Forensic Sample
For Use With (Equipment): GeneAmp™ 9700, 3130xl Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 310 Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, GeneAmp® 9600
Matrix and Size Standards: GeneScan™ 500 ROX™ Internal Lane Size Standard, Matrix Standard Set DS-32
Shipping Condition: Dry Ice

Contents & storage

• AmpFLSTR® PCR Reaction Mix (store at 2 to 8°C)
• AmpFLSTR® Profiler Plus® Primer Set (store at 2 to 8°C, protect from light)
• AmpliTaq Gold® DNA Polymerase (store at -15 to -25°C)
• AmpFLSTR® Control DNA 9947A (store at 2 to 8°C)
• Mineral oil (store at room temperature)
• AmpFLSTR® Blue Allelic Ladder (store at 2 to 8°C, protect from light)
• AmpFLSTR® Green II Allelic Ladder (store at 2 to 8°C, protect from light)
• AmpFLSTR® Yellow Allelic Ladder (store at 2 to 8°C, protect from light)


Manuals & protocols