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Applied Biosystems™

TaqMan™ Universal PCR Master Mix

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TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time.

• Contains AmpliTaq Gold DNA Polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq DNA Polymerase
• Includes a passive internal reference based on proprietary ROX dye, for superb precision on Applied Biosystems real-time PCR instruments
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
• AmpErase UNG protects against subsequent re-amplification from PCR products containing dU to minimize carry-over contamination
• Rapid assay development guidelines are provided to minimize optimization time

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: TaqMan™
Product Size: 1 x 5 mL
Sample Type (Specific): DNA (Genomic), cDNA

Contents & storage

Supplied at 2X concentration. The mix contains AmpliTaq Gold® DNA Polymerase, Uracil-DNA Glycosylase, dNTPs with dUTP, Passive Reference 1 and optimized buffer components. This pack contains one 5 mL tube, sufficient for 200 reactions at the 50 uL total volume. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols