AmpFLSTR® SGM Plus® PCR Amplification Kit

Catalog number: 4307133

Applied Biosystems™  Related applications: PCR Amplification for Human Identification

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Description

The AmpFLSTR® SGM Plus® PCR Amplification Kit amplifies 10 STR loci and the gender marker Amelogenin yielding a highly informative kit for human identification, with a 1 in 5 X 1012 average probability of identity.

Key Features of the AmpFLSTR® SGM Plus® PCR Amplification Kit:

• Ten tetranucleotide STR loci and Amelogenin are co-amplified in a single PCR tube for ease of use and increased throughput
• Amplicon allele size ranges less than 360 base pairs ensure robust PCR amplification for all loci
• Conveys certain rights to perform research and forensic testing services when used with an authorized thermal cycler
• Conveys the PCR service rights for paternity testing

More Information
Six of the STR loci (D21, FGA, TH01, vWA, D8, D18) were developed and popularized by the Forensic Science Service (FSS) in the Second Generation Multiplex (SGM) system. To create the AmpFLSTR® SGM Plus® kit, a PCR multiplex was developed and optimized to included the six core SGM loci and Amelogenin, plus four additional STR loci (D3, D19, D16, D2). The AmpFLSTR® SGM Plus® kit significantly enhances the information obtained from a single PCR.

Reliable Results
The AmpFLSTR® SGM Plus® kit has been subjected to rigorous quality-control testing to ensure reliable performance. Experiments have been performed based upon the TWGDAM recommended guidelines (Technical Working Group on DNA Analysis Methods. 1995. Guidelines for a Quality Assurance Program for DNA Analysis. Crime Lab Digest 22:21 to 43).

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For Forensic or Paternity Use Only.

Specifications

Technique: STR (Short Tandem Repeat) Analysis
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: NED™, ROX™, FAM™, JOE™
Product Size: 100 reactions
Amplicon Size: <360 bp
Sample Volume: 50 µl⁄reaction
Reaction Format: SuperMix or Master Mix
Sample Type (Specific): DNA (Genomic), Forensic Sample
For Use With (Equipment): GeneAmp® 9700, 3130xl Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 310 Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, GeneAmp® 9600
Matrix and Size Standards: GeneScan™ 500 ROX™ Internal Lane Size Standard, Matrix Standard Set DS-32
Shipping Condition: Dry Ice

Contents & storage

• PCR Reaction Mix (store at 2 to 8°C)
• SGM Plus® ID Primer Set (store at 2 to 8°C, protect from light)
• AmpliTaq Gold® DNA Polymerase (store at -15 to -25°C)
• Control DNA (store at 2 to 8°C)
• SGM Plus® Allelic Ladder (store at 2 to 8°C, protect from light)

Documents

Manuals & protocols