This product was discontinued on December 30, 2016 and no new stock is being manufactured. Remaining stock will be continued to be sold until depletion. For assistance, please contact your local Human Identification representative or HID Tech Support. Read discontinuation notice »

AmpFLSTR® SGM Plus® PCR Amplification Kit

Catalog number: 4307133

Applied Biosystems™  Related applications: Forensics

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The AmpFLSTR® SGM Plus® PCR Amplification Kit amplifies 10 STR loci and the gender marker Amelogenin yielding a highly informative kit for human identification, with a 1 in 5 X 1012 average probability of identity.

Key Features of the AmpFLSTR® SGM Plus® PCR Amplification Kit:

• Ten tetranucleotide STR loci and Amelogenin are co-amplified in a single PCR tube for ease of use and increased throughput
• Amplicon allele size ranges less than 360 base pairs ensure robust PCR amplification for all loci
• Conveys certain rights to perform research and forensic testing services when used with an authorized thermal cycler
• Conveys the PCR service rights for paternity testing

More Information
Six of the STR loci (D21, FGA, TH01, vWA, D8, D18) were developed and popularized by the Forensic Science Service (FSS) in the Second Generation Multiplex (SGM) system. To create the AmpFLSTR® SGM Plus® kit, a PCR multiplex was developed and optimized to included the six core SGM loci and Amelogenin, plus four additional STR loci (D3, D19, D16, D2). The AmpFLSTR® SGM Plus® kit significantly enhances the information obtained from a single PCR.

Reliable Results
The AmpFLSTR® SGM Plus® kit has been subjected to rigorous quality-control testing to ensure reliable performance. Experiments have been performed based upon the TWGDAM recommended guidelines (Technical Working Group on DNA Analysis Methods. 1995. Guidelines for a Quality Assurance Program for DNA Analysis. Crime Lab Digest 22:21 to 43).

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For Forensic or Paternity Use Only.


Amplicon Size: <360 bp
For Use With (Equipment): 310 Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, GeneAmp® 9600, GeneAmp™ 9700
Label or Dye: FAM™, JOE™, NED™, ROX™
Matrix and Size Standards: GeneScan™ 500 ROX™ Internal Lane Size Standard, Matrix Standard Set DS-32
Polymerase: AmpliTaq Gold® DNA Polymerase
Product Line: AmpFLSTR®, SGM Plus®
Product Size: 100 reactions
Reaction Format: SuperMix or Master Mix
Sample Type (Specific): DNA (Genomic), Forensic Sample
Sample Volume: 50 µL⁄reaction
Technique: STR (Short Tandem Repeat) Analysis
Shipping Condition: Dry Ice

Contents & storage

• PCR Reaction Mix (store at 2 to 8°C)
• SGM Plus® ID Primer Set (store at 2 to 8°C, protect from light)
• AmpliTaq Gold® DNA Polymerase (store at -15 to -25°C)
• Control DNA (store at 2 to 8°C)
• SGM Plus® Allelic Ladder (store at 2 to 8°C, protect from light)


Manuals & protocols