dGTP BigDye® Terminator v1.0 Ready Reaction Cycle Sequencing Kit

Catalog number: 4307175

Applied Biosystems™  Related applications: DNA Sequencing | Sanger Sequencing

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dGTP BigDye® Terminator Cycle Sequencing Kit with the AmpliTaq® DNA Polymerase, FS. This Ready Reaction Kit uses the same dye set as the BigDye® v1.0 kits.

The dGTP BigDye® Terminator Cycle Sequencing Kit's fluorescent dideoxy terminator chemistry is optimized for sequencing through GT- and G-rich templates.

• Ensure reliable results with difficult regions and finishing applications.
• Sequence templates with challenging sequence contexts.
• Sequence problematic templates that standard chemistries cannot.

Sequence Challenging Templates
The dGTP BigDye® Terminator Kit is ideal for working with GT- and G-rich and other difficult-to-sequence templates. The dGTP BigDye® Terminator Kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples.

Optimized for GT-Rich Templates
Obtain higher success rate even with your difficult GT-rich templates. By using the same chemistries as the standard BigDye® Terminator Kits (only replacing dITP with dGTP) the dGTP BigDye® Terminator Kit provides proven performance, optimized for GT-rich and other difficult-to-sequence templates.

Easily Integrate into Your Workflow
dGTP BigDye® Terminator Kits require no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Recommended Applications: GT-Rich or Difficult Template Sequencing
Format: 8-well strips, 384-well plate, 12-strip wells, 96-well plate
Technique: Fluorescent Dye Terminator Sequencing
Product Size: 100 reactions
Template Compatibility: BAC DNA, Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), PCR Amplicons, Single Stranded DNA

Contents & storage

The kit contains the AmpliTaq® DNA Polymerase, FS, BigDye
terminators v1.0, pGEM® - 3Zf(+) Control Template,
-21 M13 Control Primer (forward), and Premixed Ready Reaction.
Store kit at -15 to -25°C.


Manuals & protocols