Informational:Try PowerTrack SYBR Green Master Mix - our newest formulation simplifies your workflow by minimizing primer optimization and reducing pipetting errors with an optional sample tracking dye.
Applied Biosystems™

SYBR™ Green PCR Master Mix

Catalog number: 4309155
Applied Biosystems™

SYBR™ Green PCR Master Mix

Catalog number: 4309155
Catalog Number
4309155
Unit Size
1 x 5 mL
Price (USD)
Availability
-
Quantity
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Unit Size
1 x 1 mL
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Unit Size
2 x 5 mL
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Unit Size
5 x 5 mL
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10 x 5 mL
Price (USD)
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Catalog Number
Unit Size
1 x 50 mL
Price (USD)
Availability
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Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
43091551 x 5 mL
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43444631 x 1 mL
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43643442 x 5 mL
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43643465 x 5 mL
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431270410 x 5 mL
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43349731 x 50 mL
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Notification of updated SYBR Green master mix formulation: PowerTrack SYBR Green Master Mix is our newest formulation designed to greatly simplify your work and deliver high sensitivity and specificity. It comes with an optional yellow dye that can be added to your DNA sample for pipetting QC. Yellow sample added to blue master mix yields green qPCR reaction. Visually confirm that your DNA sample was added to master mix. PowerTrack SYBR Green Master Mix is compatible with a wide range of primer melting temperatures (55°C to 65°C) and concentrations to minimize primer optimization work.

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SYBR Green PCR Master Mix
Everything you need for SYBR Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

  • Premixed components stored at 2–8°C significantly reduce assay setup time
  • SYBR Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability

Maximum flexibility and convenience
Applied Biosystems SYBR Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan probes are required. SYBR Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
SYBR
Format
Tube
GC-Rich PCR Performance
High
PCR Method
qPCR
Polymerase
AmpliTaq Gold DNA Polymerase
Reaction Speed
Standard
For Use With (Equipment)
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Label or Dye
SYBR Green
Passive Reference Dye
ROX (Pre-mixed)
Product Line
SYBR™
Product Type
Real Time PCR SYBR Master Mix
Quantity
1 x 5 mL
Shipping Condition
Wet Ice
Sufficient For
200 Reactions of 50 μL
For Use With (Application)
Gene Expression
Concentration
2X
No. of Reactions
200 Reactions
Sample Type
DNA (Genomic), cDNA
Volume
1 x 5 mL

Contents & Storage

The 1 x 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 200 reactions based on a 50 μl reaction volume.

Store at -20°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

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