• Find Real-Time and Digital PCR technical tips, troubleshooting help, and resources within our new Real-Time PCR and Digital PCR Applications Support Center.

TaqMan® Universal PCR Master Mix, no AmpErase® UNG

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time.
• Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase
• Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® real-time PCR instruments.
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
• Contains dUTP in the dNTP mix to protect against carry-over contamination, when used with AmpErase® UNG
• Rapid assay development guidelines are provided to minimize optimization time
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, GeneAmp® 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold® DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: TaqMan™
Product Size: 200 reactions
Sample Type (Specific): DNA (Genomic), cDNA
Shipping Condition: Wet Ice

Contents & storage

Supplied at 2X concentration. The mix contains AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1 and optimized buffer components. This pack contains one 5 mL tube, sufficient for 200 reactions at the 50 μL total volume. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Documents

Manuals & protocols

Recommended products