I am looking for a high-fidelity enzyme (with proofreading potential) for use in PCR that does not use antibodies for the hot start.
GeneAmp High Fidelity PCR System (Cat. No. 4328216) provides the proofreading activity you require. Please note that the AmpliTaq Gold DNA polymerase enzyme is a chemically modified enzyme, and its hot-start properties are not conferred by an antibody. Please refer to the GeneAmp High Fidelity PCR System product information.