Notice: This product will be discontinued on September 1, 2017. For superior performance, we recommend Platinum™ SuperFi™ Green DNA Polymerase.

GeneAmp® High Fidelity PCR System

Catalog number: 4328217

Applied Biosystems™  Related applications: PCR

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The Applied Biosystems® GeneAmp® High Fidelity PCR System is designed for accuracy and increased PCR yield.

• AmpliTaq® DNA Polymerase-based enzyme blend is formulated for increased fidelity and yield of PCR products
• Proofreading activity enables amplification of DNA fragments up to 10 kb
• Two-buffer system provides versatility

High Fidelity
The GeneAmp® High Fidelity PCR System contains an enzyme blend of AmpliTaq® DNA Polymerase and a new thermo stable proofreading enzyme. This proprietary blend increases the fidelity of PCR 3-fold over Taq DNA Polymerase. The careful blending of these enzymes prevents truncated products resulting from misincorporated nucleotides, and limits primer degradation by the 3' to 5' exonuclease activity of the proofreader. The increased sensitivity of the unique GeneAmp® High Fidelity Enzyme Mix enables detection down to 1 ng of complex templates such as human genomic DNA.

Flexible Kit
The system is supplied with buffers specifically designed to enhance fidelity, sensitivity, and yield of PCR experiments. Two versions of the buffer are included for flexibility. For ease of use, one version contains a final MgCl2concentration of 1.5 mM. The second buffer does not contain MgCl2. A separate MgCl2 solution is also provided for optimizing MgCl2 concentration. The system allows for amplification of products ranging from a hundred bases up to 10 kb from human genomic DNA. It can be used with templates from various sources such as mammalian genomic DNA, artificial chromosomes, plasmids, and cDNA.

See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: AmpliTaq® DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 3 X
Reaction Format: Separate Components
Product Overhang: Blunt
Reaction Speed: Standard
Final Product Size(s): 10 kb or less
Exonuclease Activity: 3' - 5', 5' - 3'
Product Line: GeneAmp®
Product Size: 200 reactions

Contents & storage

Contains GeneAmp® High Fidelity Enzyme Mix, 10X PCR Buffer with MgCl2, 10X PCR Buffer without MgCl2, and MgCl2 solution. Sufficient for 200 PCR amplifications, 50 µl each. Store at –20°C.


Manuals & protocols

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