Applied Biosystems™

ABI PRISM™ 7000 Sequence Detection Systems Spectral Calibration Kit

Catalog number:  4328895

Related applications:

Real Time PCR (qPCR)

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Description

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™, ROX™, SYBR® Green, VIC™, and TAMRA™ dyes on the ABI PRISM® 7000 Sequence Detection System. The ABI PRISM® 7000 Sequence Detection Systems Spectral Calibration Kit includes eight optical 96-well reaction plates: one preloaded and sealed background plate, and seven preloaded and sealed spectral calibration plates with seven separate dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM™, JOE™, NED™, ROX™, SYBR® Green, VIC™, and TAMRA™) are provided for quick and easy calibration of the ABI PRISM® 7000 Sequence Detection System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions Spend Your Time on Research—Not Instrument Calibration This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the ABI PRISM® 7000 Sequence Detection System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
Label or Dye: FAM, JOE, NED, ROX, TAMRA™, TMR (Tetramethylrhodamine), VIC, SYBR™ Green
Product Line: ABI PRISM™
Shipping Condition: Dry Ice
Calibration Type: Spectral Calibration
For Use With (Application): Real Time PCR (qPCR)
Includes: 8 Reaction Plates
For Use With (Equipment): 7000 System
Product Type: Spectral Calibration Kit

Contents & storage

Contains one Background plate, and seven Spectral Calibration plates with seven dye standards (FAM™, JOE™, NED™,ROX™, SYBR™ Green, VIC™, TAMRA™). Store at -15 to 25°C. Protect from light.