BigDye® Terminator v1.1 Cycle Sequencing Kit

Catalog number: 4337452

Applied Biosystems™  Related applications: DNA Sequencing | Sanger Sequencing

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This BigDye® Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

• Improve the quality of your results for a wide range of sequencing applications.
• Sequence challenging templates and sequences more successfully.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Tackle Tough Sequences
With the BigDye Terminator v1.1 Cycle Sequencing Kit you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye Terminator v1.1 kit's superior chemistry, you generate data with uniform peak heights and optimized signal balance-which results in longer, higher-quality reads. You also get more accurate base assignments for heterozygote and mutation detection.

Easily Integrate into Your Workflow
BigDye® Terminator v.1.1 kit's chemistry requires no new software or instrument recalibration.You can easily integrate either kit into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): GeneAmp™ 9700, Veriti® Thermal Cycler, 3500xL Genetic Analyzer, 3500 Genetic Analyzer, 3130xl Genetic Analyzer, 310 Genetic Analyzer, 3130 Genetic Analyzer, 3730xl DNA Analyzer, 3730 DNA Analyzer
Recommended Applications: Standard Sequencing
Format: 8-well strips, 12-strip wells, 96-well plate, 384-well plate
Technique: Fluorescent Dye Terminator Sequencing
Product Size: 5,000 reactions
Template Compatibility: BAC DNA, Single Stranded DNA, PCR Amplicons, Plasmid DNA (≤15Kb)

Contents & storage

Each kit contains 2 × 20-ml tubes of BigDye® Terminator v1.1 Ready Reaction Mix, 2 tubes of M13 (-21) Primer, 2 tubes of pGEM Control DNA, one 28-mL tube of 5X Sequencing Buffer (may be stored at 4°C).

Store kit at -15°C to -25°C and avoid repeated freeze-thaw cycles.


Manuals & protocols