Applied Biosystems™

BigDye™ Terminator Plus Cycle Sequencing Kit

Name: BigDye™ Terminator Plus Cycle Sequencing Kit
SKU: 4337461
Price: 449.00 EUR

The BigDye™ Terminator BDT Plus Cycle Sequencing Kit is a Sanger sequencing master mix designed to enable faster results and improved performance across a broad range of sample types.

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Catalog Number	Quantity
4337461	192 μL
4337462	800 μL
4337463	8 mL
4337465	40 mL
4337466	200 mL

5 Options

Catalog number 4337461

Price (EUR)

449,00

Each

Quantity:

192 μL

Price (EUR)

449,00

Each

The BigDye™ Terminator BDT Plus Cycle Sequencing Kit is a Sanger sequencing master mix designed to enable faster results and improved performance across a broad range of sample types. Built on a newly developed high-performance enzyme, BDT Plus enables more efficient sequencing of challenging templates while maintaining full compatibility with existing BigDye™ workflows and capillary electrophoresis (CE) instruments. Compared to legacy BigDye™ Terminator v3.1 chemistry, BDT Plus helps provide enhanced speed, flexibility, and reliability without requiring workflow changes.

Key Features and Benefits

Accelerate sequencing workflows with up to ~50% faster time to results
Improve performance on challenging templates using an advanced high-processivity enzyme
Maintain existing workflows with full compatibility across current BigDye™ protocols and CE instruments
Increase success rates and reduce re-runs for greater laboratory efficiency
Flexible Protocol Optimization: Supports implementation of customer-optimized workflows, allowing laboratories to maintain validated protocols and specialized applications

Product Details

The BDT Plus kit incorporates a newly engineered enzyme with increased processivity, allowing for more efficient incorporation of dye-labeled terminators during cycle sequencing. This enhanced enzyme performance improves read quality and consistency, particularly when working with difficult templates such as GC-rich regions, secondary structures, or low-quality samples. The optimized formulation supports robust sequencing across a wider range of sample inputs and conditions.

BDT Plus is designed as a drop-in replacement for existing BigDye™ Terminator chemistries, requiring no changes to established workflows. Researchers can integrate the kit directly into their current cycle sequencing protocols, using the same instruments, plastics, and cleanup methods. This compatibility reduces disruption while enabling immediate performance improvements.

The kit supports a wide range of Sanger sequencing research applications, including assay development, mutation detection, plasmid verification, and confirmatory sequencing. It is well suited for use in clinical research, biopharma, academic, and core laboratory environments, where sample diversity and throughput requirements vary. Its ability to process challenging and heterogeneous sample types makes it particularly valuable for labs seeking consistent results across complex workflows.

By combining faster turnaround times with improved sequencing performance, BDT Plus helps laboratories increase productivity, reduce repeat runs, and maintain confidence in their sequencing data.

For Research Use Only.

Specifications

For Use With (Application)Sanger Sequencing, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing

For Use With (Equipment)SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers

Product LineBigDye Terminator

Product TypeSequencing Kit

Quantity192 μL

TechniqueFluorescent Dye Terminator Sequencing

Template CompatibilityBAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons

FormatTube

Isolation TechnologyMagnetic Bead

Sequencing TypeGenome and DNA Sequencing

Unit SizeEach

Frequently asked questions (FAQs)

Can I dilute sequencing reactions when using BigDye Terminator Plus Cycle Sequencing Kit?

Yes, sequencing reactions can be diluted when using the BigDye Terminator Plus Cycle Sequencing Kit by using the 5X Sequencing Buffer. When reducing the Ready Reaction Mix volume, additional buffer should be added to maintain a final 1X buffer concentration.
The 5X Sequencing Buffer contains buffer components (for example, Mg²⁺ and Tris) at a higher concentration than the Ready Reaction Mix.
Specifically, the Ready Reaction Mix provides buffer at 2.5X, while the Sequencing Buffer is at 5X, meaning the buffer components are twice as concentrated in the 5X Sequencing Buffer compared to the Ready Reaction Mix. To maintain a final 1X buffer concentration in the sequencing reaction when reducing the volume of Ready Reaction Mix, the volume of 5X Sequencing Buffer added should compensate for the missing buffer. This can be calculated as:

Volume of 5X Sequencing Buffer = ½ × (Nominal Ready Reaction Mix volume – Actual Ready Reaction Mix volume)

Example: For a 10 µL sequencing reaction:
Nominal Ready Reaction Mix volume (full reaction): 4 µL
Actual Ready Reaction Mix volume used: 1 µL
Volume of 5X Buffer = ½ × (4 µL – 1 µL)
Volume of 5X Buffer = ½ × 3 µL = 1.5 µL
In this example, adding 1.5 µL of 5X Sequencing Buffer compensates for the reduced Ready Reaction Mix volume and maintains the correct final buffer concentration for optimal sequencing performance.
This helps to ensure that the overall buffer composition remains consistent despite dilution of the sequencing chemistry.

Can BigDye Terminator Plus Cycle Sequencing Kit be used with dGTP chemistry?

Yes, BigDye Terminator Plus Cycle Sequencing Kit can be used with dGTP chemistry when sequencing challenging templates. When using dGTP, standard reaction setups can be followed, with the addition of a small proportion of dGTP BigDye master mix to the sequencing reaction. Optimization may be required depending on the template.

Is BigDye Terminator Plus Cycle Sequencing Kit compatible with sequencing additives such as DMSO or betaine?

Yes, BigDye Terminator Plus Cycle Sequencing Kit is compatible with commonly used sequencing additives, including GC enhancers, DMSO, betaine, sulfolane, and sequencing enhancer solutions. These additives may be used when working with particularly challenging templates.

How does BigDye Terminator Plus Cycle Sequencing Kit perform on challenging templates such as GC-rich regions?

BigDye Terminator Plus Cycle Sequencing Kit is designed to support sequencing performance across challenging templates, including GC-rich regions, repetitive sequences, and templates with secondary structure.

How does BigDye Terminator Plus Cycle Sequencing Kit compare to BigDye Terminator v3.1 Cycle Sequencing Kit in read length and performance?

BigDye Terminator Plus Cycle Sequencing Kit is designed to support longer read lengths and more consistent signal across challenging templates compared to BigDye Terminator v3.1 Cycle Sequencing Kit, particularly in GC-rich or structured regions.

View all BigDye™ Terminator Plus Cycle Sequencing Kit FAQs