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AmpliTaq Gold™ DNA Polymerase, LD (Low DNA) with Gold Buffer and MgCl2
AmpliTaq Gold&trade; DNA Polymerase, LD (Low DNA) with Gold Buffer and MgCl<sub>2</sub>
Applied Biosystems™

AmpliTaq Gold™ DNA Polymerase, LD (Low DNA) with Gold Buffer and MgCl2

Applied Biosystems AmpliTaq Gold DNA Polymerase, LD (Low DNA) is a highly purified version of AmpliTaq Gold DNA Polymerase that significantly reduces contaminating bacterial DNA sequences commonly present in recombinant protein preparations.
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Catalog NumberQuantity
4338856250 Units
43388571000 Units
Catalog number 4338856
Price (MXN)
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Quantity:
250 Units
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Applied Biosystems AmpliTaq Gold DNA Polymerase, LD (Low DNA), is a highly purified version of AmpliTaq Gold DNA Polymerase that significantly reduces contaminating bacterial DNA sequences commonly present in recombinant protein preparations.

This highly purified enzyme preparation gives you more confidence in your results and makes advanced PCR techniques quick and easy. When combined with the supplied GeneAmp 10X PCR Gold Buffer, AmpliTaq Gold DNA Polymerase, LD, provides a flexible hot-start system that is suitable for all PCR applications that require the elimination of bacterial DNA contamination.

Features

  • Highly purified enzyme with lower levels of bacterial DNA
  • Minimized non-target, false-positive PCR products when amplifying bacterial sequences
  • Chemically modified hot-start technique eliminates biological contamination
  • 10X PCR Gold Buffer promotes fast enzyme activation and robust PCR amplification
  • Enhanced specificity with time-release PCR for low-copy target amplification
  • Improved yield and success rate for target amplification

Notes

  • AmpliTaq Gold DNA Polymerase, LD, is recommended for PCR applications that require low background levels of bacterial DNA.
  • See user's manual or package insert for limited label license and trademark information.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatTube
Hot StartBuilt-In Hot Start
No. of Reactions200 Reactions
Overhang3'-A
PolymeraseAmpliTaq Gold DNA Polymerase
Product TypeDNA Polymerase
Purity or Quality GradeLD (Low DNA)
Quantity250 Units
Reaction FormatSeparate Components
Shipping ConditionDry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
Concentration5 U/μL
For Use With (Application)Hot-start PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• AmpliTaq Gold DNA Polymerase, LD (5 U/μL), 1 x 50 μL
• 10X PCR Gold Buffer, 1 x 1.5 mL
• 25 mM MgCl2, 1 x 1.5 mL

Store at -15°C to -30°C.

Frequently asked questions (FAQs)

What is the difference between AmpliTaq Gold polymerase and Platinum Taq polymerase?

Both AmpliTaq Gold and Platinum Taq are hot-start enzymes that allow you to set up your reactions on the benchtop without the need for ice. AmpliTaq Gold is a chemically-modified hot-start enzyme, provided in an inactive state. Heat activates the enzyme, with full activity after 10 min at 95 degrees C. Platinum Taq is an antibody-mediated hot-start enzyme. The anti-Taq antibodies bind and inactivate the enzyme, until the heat denaturation step of the PCR reaction (30 sec to 2 min), which activates the enzyme.

When using AmpliTaq Gold DNA polymerase, under what conditions would one choose a two-temperature vs. three-temperature PCR?

A two-temperature PCR is commonly used when the primer annealing temperatures are above 60 degrees C. Use a three-temperature PCR when the templates have high G+C content and/or secondary structure, or desired primer annealing temperatures are below 60 degrees C. Please consult the Product Insert for more information on the use of AmpliTaq Gold DNA polymerase.

Is there anything to prevent AmpliTaq Gold DNA polymerase from extending from the 3’ end of a TaqMan probe in a 5’ nuclease assay?

Yes. There is a phosphate group on the 3' end of all TaqMan probes that prevents such extension.

How does AmpliTaq Gold DNA Polymerase differ from AmpliTaq DNA Polymerase?

AmpliTaq Gold DNA Polymerase is a modified form of AmpliTaq DNA Polymerase that contains a proprietary chemical (or so-called hot start molecule) bound to the enzyme's active site. In order to activate the AmpliTaq Gold DNA Polymerase fully, we recommend an initial activation step of 95 degrees C for 10 min when using GeneAmp 10X PCR Buffer I and/or GeneAmp 10X PCR Buffer II and Mg in one of our thermal cyclers. When using GeneAmp 10X PCR Gold Buffer, activation time can be reduced to 5 minutes. Once activation is complete, you can proceed with your standard PCR cycling program (denaturing, annealing, extension, etc).

Does AmpliTaq Gold DNA Polymerase contain exonuclease (proofreading) activity?

No, AmpliTaq Gold DNA polymerase does not contain proofreading activity, however fidelity in PCR amplifications utilizing this enzyme may be improved. High fidelity can be achieved by: 1. Decreasing the final concentration of each nucleotide to 40-50 uM. 2. Using the lowest MgCl2 concentration possible. 3. Using less enzyme. 4. Decreasing extension times. 5. Using the highest annealing temperature possible. 6. Using as few cycles as possible.

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