Quantifiler® Y Human Male DNA Quantification Kit

Catalog number: 4343906

Applied Biosystems™  Related applications: Data Interpretation for Human Identification

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This real-time PCR-based DNA quantification kit is validated for human identification applications. It quantifies the amount of amplifiable human male DNA that is present in a sample. An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors.

• Quantifies male DNA from a wide variety of sources including buccal swabs, blood, semen, and tissue, making this kit suitable for forensic and paternity laboratories.
• Easily integrates into current workflow.
• Internal PCR Control (IPC) allows for quick monitoring of amplification success and identifies samples that may contain inhibitors.
• Real-time PCR offers the widest dynamic range of any DNA detection technology to accurately quantify low levels of samples. Quantifies a broad range of DNA concentrations from 0.023 ng⁄ml to>50 ng⁄ml.

Maximize the Information Retrieved from Mixed Sexual Assault Samples
The kit helps detect male DNA from samples with male⁄female mixtures with a DNA ratio greater than 1:1000 male⁄female DNA. In instances of samples with male⁄female mixtures, Quantifiler® Y Male Human DNA Quantification Kit helps you to choose the optimal kit for short tandem repeat (STR) analysis. It also helps you more accurately determine the amount of sample input into your STR amplification reaction.

Easy to Use, Single Tube Format
PCR reaction and quantification performed in single tube helping to minimize contamination.

Trusted TaqMan® Assays
TaqMan® assays are used throughout the scientific community for scientific assays including: gene expression, DNA quantification, presence or absence of a nucleic acid, and SNP genotyping assays. The Quantifiler® Y kits utilize this widely known and trusted technology for the quantification of male DNA (see figure at right).

Experience Matters
When you partner with us you gain access to the extensive knowledge and experience of our forensic support team and training resources. Our HID University provides continuing education for DNA professionals, our support and Applications Scientist offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.

Used With
Quantifiler® Human DNA Quantification Kit, Quantifiler® Human DNA Quantification Kits User’s Manual.

For Research Use Only. Not for use in diagnostics procedures.
For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.


PCR Method: qPCR
Target Gene: Sex-determining region Y gene (SRY)
Product Size: 400 reactions
Amplicon Size: 64 bases
Detection Method: Primer-Probe Detection
Sample Type (Specific): DNA (Genomic), Forensic Sample, Forensic Sample (Highly Degraded)
Detection Range or Limit: Can reproducibly detect <16 pg⁄µl of human genomic DNA
For Use With (Equipment): 7000 System, 7500 System
Shipping Condition: Dry Ice

Contents & storage

This kit includes Quantifiler® PCR Mix, Quantifiler® Male Primer Mix, and the Quantifiler® DNA Standard.

Store the PCR Mix at 2 - 6°C.

Store the Quantifiler® DNA Standard at -15° to -25°C.

Store Quantifiler® Human Primer Mix a -15° to -25°C away from light.


Manuals & protocols