SYBR® Green PCR Master Mix

Catalog number: 4344463

 Related applications: Real Time PCR (qPCR)

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The SYBR® Green PCR Master Mix, optimized for real-time PCR analysis, conveniently combines SYBR Green 1 Dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer components in easy-to-use premix vials.

• Easy-to-use solution contains all components needed for PCR applications using SYBR® Green dye, which minimizes assay set-up time
• Used with rapid assay development guidelines, that minimize optimization time
• SYBR® Green I dye detects double-stranded DNA, so specific probes are not required
• Includes Passive Reference I, which is required for signal normalization in all SYBR® Green dye reactions
• Contains AmpliTaq Gold® DNA polymerase, which allows the highest performance available by minimizing non-specific product formation (including primer-dimer)
• Optimized buffer components are proprietary buffer enhancements that add to the performance and reliability of the assay using SYBR® Green dye
• Recommended storage conditions are 2°-8°C, so no thawing is required before use, thereby minimizing assay set-up time

Applied Biosystems has developed a comprehensive set of guidelines to ensure success when using sequence detection systems (SDS) reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

All SDS reagent kits contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that result from a variety of causes, such as pipetting errors and sample evaporation.

Real-time quantitative PCR was first performed using the 5' fluorogenic nuclease assay, which uses a TaqMan® probe during the PCR amplification to provide added specificity. An additional chemistry is also available, using SYBR® Green I dye, that can provide real-time quantitative PCR information. Assays using the SYBR® Green 1 double-stranded DNA binding dye do not require a TaqMan® probe and provide additional experimental flexibility.

The incorporation of SYBR Green I dye into a real-time PCR reaction lets the user detect any double-stranded DNA generated during PCR. This provides great flexibility because no target specific probes are required, and yet both specific and non-specific products will generate signal. The use of the hot-start enzyme AmpliTaq Gold® DNA Polymerase in all SYBR Green I reagent kits yields the highest performance available by minimizing non-specific product formation. SYBR Green I dyes are ideal for use in target identification (screening assays), or when only a small number of reactions are required for a given assay.

For Research Use Only. Not for use in diagnostics procedures.


Detection Method: SYBR
For Use With (Equipment): 7700 System, 7900HT System, 7500 System, StepOnePlus™, Standard Mode, QuantStudio™, 7000 System, 7300 System, ViiA™ 7 System, StepOne™, Standard Mode
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Form: Concentrated
Hot Start: Built-In Hot Start
Technique: Fluorescence Intensity
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: SYBR® Green
Product Size: 1 x 1 mL
Shipping Condition: Wet Ice
Target Specificity: Not Target-Specific
Number of Reactions: 40 Reactions
Passive Reference Dye: ROX (Pre-mixed)
Primer-Probe Compatibility: Unlabeled Oligos

Contents & storage

SYBR® Green PCR Master Mix in a 1 mL vial. Sufficient reagents provided for 40 reactions based on a 50 µl reaction volume. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols

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