MicroSEQ™ 500 16S rDNA Sequencing Kit
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Applied Biosystems™

MicroSEQ™ 500 16S rDNA Sequencing Kit

The MicroSEQ™ 500 16S rDNA Sequencing Kit is the sequencing component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System,Read more
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Catalog NumberQuantity
43464801 Kit
Catalog number 4346480
Price (USD)
612.00
Each
Add to cart
Quantity:
1 Kit
Price (USD)
612.00
Each
Add to cart
The MicroSEQ™ 500 16S rDNA Sequencing Kit is the sequencing component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. Use this kit to sequence PCR products that have been generated using the MicroSEQ™ 500 16S rDNA PCR Kit. The two kits together permit the quick and convenient amplification and sequencing of 500 base pairs of the 16S rRNA gene. Sequences can then be compared to the MicroSEQ™ 16S rDNA 500 Library to generate an identity match. This kit does not include a protocol or a Quick Reference Card.

• Sequence-based measurement ensures you get reproducible results in under 24 hours.
• Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ™ ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training-same work flow as the MicroSEQ™ D2 LSU fungal kit.

The MicroSEQ™ ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems™ MicroSEQ™ System combines the advantages of MicroSEQ™ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSeq bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostics procedures.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodCapillary Electrophoresis
For Use With (Application)Sequencing
For Use With (Equipment)310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
IncludesForward sequencing mix (2 tubes), and reverse sequencing mix (2 tubes)
LibrariesCustomizable, Bacterial Library (> 2000 species)
No. of Reactions55 Forward, 55 Reverse
Product LineMicroSEQ™
Product TypeSequencing Kit
Quantity1 Kit
Sample TypePCR Products
Shipping ConditionDry Ice
SoftwareMicroSEQ™ ID Analysis Software
Target1st 500 bp of 16S rRNA gene
Target Organism ClassBacteria
TechniqueSBT (Sequence-Based Typing)
Workflow StepCycle Sequencing
FormatKit
Unit SizeEach
Contents & Storage
Each kit contains:
Two tubes each of forward and reverse sequencing mix. Sufficient reagent to perform 55 forward and 55 reverse sequencing reactions. Store at -15 to -25°C.

Frequently asked questions (FAQs)

Which thermocyclers are compatible with the MicroSEQ 500 16S rDNA Sequencing Kit?

To take advantage of the reduced amplification and sequencing times allowed by the new fast PCR chemistry and the 9800 Fast Thermal Cycler, we recommend using the Applied Biosystems 9800 Fast Thermal Cycler with the MicroSEQ kits. The GeneAmp PCR System 9700 may also be used but note that the 9700 thermocycler does not provide the reduced amplification and sequencing times allowed by the new fast PCR chemistry and the 9800 Fast Thermal Cycler.

Do you have stability information for the MicroSEQ 500 16S rDNA Sequencing Kit (Cat. No. 4346480) when subjected to temperature fluctuations?

The MicroSEQ 500 16S rDNA Sequencing Kit is stable if stored at -20 degrees C for 15-16 months. We do not have any information available for temperatures outside of this range.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

Is the MicroSEQ 500 16S rDNA Sequencing Kit (Cat. No. 4346480) FDA approved?

No, it is for RUO.