Applied Biosystems™

MicroSEQ™ 500 16S rDNA Sequencing Kit

Catalog number:  4346480

 Related applications: Microbial Identification & Detection | Microbial Protein Production

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The MicroSEQ® 500 16S rDNA Sequencing Kit is the sequencing component of the MicroSEQ® 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. Use this kit to sequence PCR products that have been generated using the MicroSEQ® 500 16S rDNA PCR Kit. The two kits together permit the quick and convenient amplification and sequencing of 500 base pairs of the 16S rRNA gene. Sequences can then be compared to the MicroSEQ® 16S rDNA 500 Library to generate an identity match. This kit does not include a protocol or a Quick Reference Card.

• Sequence-based measurement ensures you get reproducible results in under 24 hours.
• Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ® ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training-same work flow as the MicroSEQ® D2 LSU fungal kit.

The MicroSEQ® ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems® MicroSEQ® System combines the advantages of MicroSEQ® ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSeq bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Target Organism Class: Bacteria
Target Gene: 1st 500 bp of 16S rRNA gene
Validated Application: Sequencing
Detection Method: Capillary Electrophoresis
For Use With (Equipment): 310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp 9700
Sample Type (Specific): PCR Products
Technique: SBT (Sequence-Based Typing)
Library Type: Bacterial Library (> 2000 species), Customizable
Analysis Software: MicroSEQ® ID Analysis Software
Product Line: MicroSEQ™
Product Size: 1 kit
Shipping Condition: Dry Ice
Target Molecule: DNA
Workflow Step: Cycle Sequencing

Contents & storage

Each kit contains:
Two tubes each of forward and reverse sequencing mix. Sufficient reagent to perform 55 forward and 55 reverse sequencing reactions. Store at -15 to -25°C.


Manuals & protocols

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