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Applied Biosystems™

MicroSEQ™ D2 rDNA Fungal Sequencing Kit

Catalog number:  4347481

 Related applications:

Microbial Identification & Detection

| Microbial Protein Production

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Description

This kit is the sequencing component of the MicroSEQ® Fungal Identification System, which provides an easy-to-use DNA sequence-based method to identify most fungi. It includes the primers needed to sequence the PCR products generated using the PCR component. A protocol and Quick Reference Card are not included.
• Sequence-based measurement ensures you get reproducible results in less than 24 hours.
• Growth requirement-independent measurement enables you to identify fungi using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ® ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training; same work flow as the MicroSEQ® Full Gene 16S rDNA Bacterial Identification Kit.

Specific and Easy to Use
Based on phylogenetic analysis of the D2 region of large-subunit ribosomal RNA gene sequences, this easy-to-use kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens. To ensure accurate and specific results, the kit's software compares your samples to a database of D2 sequences from over 1,000 validated type species.

The MicroSEQ® ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems® MicroSEQ® System combines the advantages of MicroSEQ® ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ® bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostics procedures.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Target Organism Class: Fungi
Target Gene: D2 region of rRNA gene
Validated Application: Sequencing
Detection Method: Capillary Electrophoresis
For Use With (Equipment): 310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, 9800 Fast Thermal Cycler, GeneAmp 9700, Veriti Thermal Cycler
Sample Type (Specific): PCR Products
Technique: SBT (Sequence-Based Typing)
Library Type: Customizable, Fungal Library (> 1100 species)
Analysis Software: MicroSEQ® ID Analysis Software
Product Line: MicroSEQ™
Product Size: 1 kit
Shipping Condition: Dry Ice
Target Molecule: DNA
Workflow Step: Cycle Sequencing

Contents & storage

The kit contains reagents sufficient for 55 sequencing reactions:
D2 Forward Sequencing Mix (store at -15 to -25°C)
D2 Reverse Sequencing Mix (store at -15 to -25°C)

Documents

Manuals & protocols