Notice of format change: Tube cap and label colors for reverse sequencing mix have been changed to yellow to distinguish reagent from forward sequencing mix. Read entire notice »
Applied Biosystems™
MicroSEQ™ D2 rDNA Fungal Sequencing Kit
Error loading your content!
| Catalog number |
Select a plan
Unit size
|
Price ({{currency}}) | Availability | Qty | |
|---|---|---|---|---|---|
| {{product.sku}} {{product.sku}} also known as {{product.formattedSku}} |
|
— |
Pro add-ons
Your on-site stock
››
{{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
››
{{supplyCenter.scName}}(Out of stock)
››
{{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Description
This kit is the sequencing component of the MicroSEQ® Fungal Identification System, which provides an easy-to-use DNA sequence-based method to identify most fungi. It includes the primers needed to sequence the PCR products generated using the PCR component. A protocol and Quick Reference Card are not included.• Sequence-based measurement ensures you get reproducible results in less than 24 hours.
• Growth requirement-independent measurement enables you to identify fungi using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ® ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training; same work flow as the MicroSEQ® Full Gene 16S rDNA Bacterial Identification Kit.
Specific and Easy to Use
Based on phylogenetic analysis of the D2 region of large-subunit ribosomal RNA gene sequences, this easy-to-use kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens. To ensure accurate and specific results, the kit's software compares your samples to a database of D2 sequences from over 1,000 validated type species.
The MicroSEQ® ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems® MicroSEQ® System combines the advantages of MicroSEQ® ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ® bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.
For Research Use Only. Not for use in diagnostics procedures.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Target Organism Class: | Fungi |
|---|---|
| Target Gene: | D2 region of rRNA gene |
| Validated Application: | Sequencing |
| Detection Method: | Capillary Electrophoresis |
| For Use With (Equipment): | 310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, 9800 Fast Thermal Cycler, GeneAmp 9700, Veriti Thermal Cycler |
| Sample Type (Specific): | PCR Products |
| Technique: | SBT (Sequence-Based Typing) |
| Library Type: | Customizable, Fungal Library (> 1100 species) |
| Analysis Software: | MicroSEQ® ID Analysis Software |
| Product Line: | MicroSEQ™ |
| Product Size: | 1 kit |
| Shipping Condition: | Dry Ice |
| Target Molecule: | DNA |
| Workflow Step: | Cycle Sequencing |
Contents & storage
The kit contains reagents sufficient for 55 sequencing reactions:
D2 Forward Sequencing Mix (store at -15 to -25°C)
D2 Reverse Sequencing Mix (store at -15 to -25°C)
D2 Forward Sequencing Mix (store at -15 to -25°C)
D2 Reverse Sequencing Mix (store at -15 to -25°C)
