This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the Applied Biosystems™ 7300 Real-Time PCR System. The Applied Biosystems™ 7300 Real-Time PCR Systems Spectral Calibration Kit I contains one background plate, one region of interest (ROI) calibration plate, and seven spectral calibration plates with seven separate dye standards.

• 96-well optical reaction plates preloaded with dyes (FAM™/SYBR™ Green I, VIC™/JOE™, NED™/TAMRA™/ROX™) enable you to calibrate the 7300 Real-Time PCR System quickly and easily
• Easy-to-use, preloaded spectral calibration plates reduce setup time
• Provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable quick and easy calibration of the 7300 Real-Time PCR System. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses this calibration information to ensure accurate data analysis.