Applied Biosystems™

TaqMan™ SNP Genotyping Assay, human This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  4351376

Related applications:

Genotyping & Genomic Profiling

| Real Time PCR (qPCR)

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Description

Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.

Our predesigned TaqMan SNP Genotyping Human Assays are a genome-wide collection of millions of human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNPs.

Benefits:
Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
Relevant—extensive collection of predesigned human assays offers direct access to content that is relevant to your research
Tested—all human SNP genotyping assays are functionally tested to ensure allelic discrimination

Approximate ship time
4–6 days in North America and 6–10 days in Europe

TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).

All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix (sold separately): TaqMan Genotyping Master Mix

*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

3' Primer Modification: None
5' Primer Modification: None
CTA Type: Magellan_PDP_OrderNow
Comments: FAM - MGB probe detects Allele 2,VIC® - MGB probe detects Allele 1
Concentrated: 40 X
Form: Frozen
Green Features: Sustainable packaging
Internal Probe Modification: FAM (5'),MGB (Minor Groove Binder) (3'),VIC® (5')
Label or Dye: FAM,VIC
Product Line: TaqMan™
Product Size: M (1000 reactions), Made to Order
Purification: Solid Phase Extraction (SPE)
Purity or Quality Grade: DNase-Free,RNase-Free
Shipping Condition: Room Temperature
Species: Human
Target Gene: Over 4.5 million genome-wide human assays are available

Contents & storage

1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers).

Store at -15 to -25°C.