The 7900HT System Fast 96-Well Block Upgrade Kit can be used with the 7900HT Fast Real-Time PCR System. The FastMás información
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Número de catálogo
Cantidad
4351402
1 Kit
Número de catálogo 4351402
Precio (MXN)
-
Cantidad:
1 Kit
The 7900HT System Fast 96-Well Block Upgrade Kit can be used with the 7900HT Fast Real-Time PCR System. The Fast block decreases thermal cycling times when compared to the standard block. Customers who have an Applied Biosystems 7900HT Sequence Detection System must order a 7900HT System Fast Service Upgrade (4351412) or 7900HT System Fast Service Upgrade with Computer (4362078).
The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan™ Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability. • Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate. • Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction. • Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences. • 96- or 384-well plate compatibility (including the TaqMan™ Low Density Array). • Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security. • Hands-free plate-loading and unloading provides true walkaway automation allowing you to increase your lab's productivity. • Proven assay development guidelines save time and money
High Throughput The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. An Automation Accessory combined with 384-well plate capability make the 7900HT system ideally suited to meet the high-throughput requirements of today's drug discovery process. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.
Flexibility The 7900HT system is a versatile research tool that can accommodate any real-time PCR need. User-interchangeable thermal cycling block formats let you select the format that's right for your project, using industry-standard 96- and 384-well formats, as well as a novel 384-well TaqMan™ Low Density Array and a new Fast 96-well block that reduces run times from 2 hours to about 30 minutes. An easy automation upgrade path lets you add features to meet throughput demands. A Fast PCR option reduces run times from 2 hours to about 30 minutes.
Powerful Software Using two automation software tools, Plate Utility and Automation Controller, most of the gene expression and SNP genotyping workflow is fully automated for high-throughput applications and requires minimal user intervention. You can now process 5,000 x 384 well plates (384 markers), which equates to 1.92 million genotypes. Using Relative Quantification (RQ) Manager, you can also process 200 x 384-well plates (96 detectors with quadruplicate data points⁄multiplexed endogenous control), which equates to 153,600 data points. Fully integrated into one complete Enterprise system, SNP Manager and RQ Manager eliminate data analysis bottlenecks in high-throughput SNP and gene expression research by allowing significantly more plates to be analyzed simultaneously. This reduces hands-on analysis time and simplifies the data analysis workflow.
For Research Use Only. Not for use in diagnostics procedures.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (equipo)7900HT
Formato96-well Plate
Cantidad1 Kit
Velocidad de reacciónFast
Formato de bloqueIntercambiables
Tipo de productoBlock Upgrade Kit
Unit SizeEach
Contenido y almacenamiento
1 Kit
Preguntas frecuentes
Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?
It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system
Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".
What is the difference between a Fast 96-well and standard 96-well block?
There are two main differences between a Fast 96-well and standard 96-well block for the Applied Biosystems real-time PCR systems:
• Reaction well volume: The Fast 96-well blocks have 0.1 mL reaction wells and are compatible with 0.1 mL plates and tubes. The standard 96-well blocks have 0.2 mL reaction wells and are compatible with 0.2 mL plates and tubes.
• Ramp rate: The fast 96-well blocks have a higher maximum ramp rate than standard blocks for the same real-time PCR system. However, both the fast 96-well and standard 96-well blocks are capable of running standard and fast chemistry.
Note: For newer instruments (QuantStudio 3 and 5 Real-Time PCR Systems and later) "0.1 mL" and "0.2 mL" are being used in place of "fast" and "standard" as designations for the two 96-well block formats.
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