Applied Biosystems™

Rat ACTB (actin, beta) Endogenous Control (VIC™/MGB probe, primer limited)

Green features
Catalog number: 4352340E
Applied Biosystems™

Rat ACTB (actin, beta) Endogenous Control (VIC™/MGB probe, primer limited)

Green features
Catalog number: 4352340E
Catalog Number
4352340E
Unit Size
2500 reactions
Price (USD)
732.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4352340E2500 reactions
732.00
Your Price:
***
Product Overview
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The Applied Biosystems® Rat ACTB (actin, beta) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when it is used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: ACTB
RefSeq: NM_031144.2
Probe Exon Location:4-5
Amplicon Size: 91
Corresponding TaqMan Assay ID: Rn00667869_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number
NM_031144.2
Amplicon Size
91
For Use With (Application)
Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling
Gene Symbol
ACTB
Green Features
Sustainable packaging
Includes
Control Primer-Probe Set(s)
Label or Dye
VIC
Product Line
TaqMan™, VIC™
Shipping Condition
Room Temperature
Species
Rat
Target Gene
β-actin
Concentration
20X
Detection Method
Fluorescence
Form
Liquid
Format
Tube(s)
No. of Reactions
2500 Reactions
PCR Method
2-Step RT-PCR

Contents & Storage

Package includes 4 tubes containing 20X mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 °C to -25 °C

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