Applied Biosystems™

GeneAmp™ Fast PCR Master Mix (2X)

Catalog number:  4359187

 Related applications: PCR

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The GeneAmp® Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti® 96-Well Thermal Cycler. Features of this master mix:

• Set up reactions quickly, using premixed reagents
• Detect a single copy of a gene in as little as 10 ng of DNA
• Get reproducible, specific, and sensitive results

Easy to use
Pre-mixed, hot-start chemistries enable you to set up your experiments quickly. And by generating greater than 90% "A" overhangs—it makes cloning easier too.

Detect a single copy in 10 ng of DNA
Use the GeneAmp® Fast PCR Master Mix to detect a single copy of a gene in 10 ng of human genomic DNA or as little as 10 copies of an HIV target.

Part of the Applied Biosystems® Fast PCR System
The Applied Biosystems® Fast PCR System, consisting of the premixed GeneAmp® Fast PCR Master Mix, specially designed microplates and tubes, and the Veriti® 96-Well Thermal Cycler (0.1 mL or 0.2 mL), considerably reduces PCR reaction times.
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: AmpliTaq® DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Medium
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Fast
Final Product Size(s): 2 kb or less
Exonuclease Activity: 5' - 3'
Product Line: GeneAmp®
Product Size: 250 reactions
Shipping Condition: Dry Ice

Contents & storage

The GeneAmp® Fast PCR Master Mix (2X) contains two tubes (1.25 mL each) sufficient for 125 20µl PCR reactions. The Fast PCR Master Mix can also be ordered with a protocol.

Store at 2 to 8 °C.


Manuals & protocols