Plates and Modules with Affinity Binding Surfaces
Plates and Modules with Affinity Binding Surfaces
Thermo Scientific™

Plates and Modules with Affinity Binding Surfaces

Help reduce variability in molecular orientation with these plates and modules with affinity binding surfaces.
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產品號碼ColorProduct TypeSurface TreatmentTotal Volume (Metric) Well
436033WhiteMicroplateGlutathione400 μL
436014ClearMicroplateStreptavidin400 μL
436015WhiteMicroplateStreptavidin400 μL
436016BlackMicroplateStreptavidin400 μL
436017ClearMicroplateStreptavidin120 μL
436020ClearSolid Module F8 (x12) in FrameStreptavidin400 μL
436022ClearLock-Well Module C8 (x12) in FrameStreptavidin350 μL
95029263ClearMicroplateStreptavidin400 μL
95029293ClearBreakable Module F8 (x12) in FrameStreptavidin400 μL
產品號碼 436033
價格 (HKD)
-
申請報價
Color:
White
Product Type:
Microplate
Surface Treatment:
Glutathione
Total Volume (Metric) Well:
400 μL

Help reduce variability in molecular orientation with Thermo Scientific™ Plates and Modules with Affinity Binding Surfaces.

Affinity Capture Surfaces (Passive Streptavidin and Immobilizer Streptavidin, Nickel Chelate and Glutathione, BioBind):

  • Highly specific binding surfaces reduce variability in molecular orientation.
  • Immobilizer surfaces improve signal to noise ratios.
  • Streptavidin biotin interaction can be exploited to immobilize a wide range of biomolecules.
規格
Binding TypeAffinity
Detection MethodChemiluminescence
No. per Case15
No. per Pack1
Packaging15 Cases
Plate BlockingUnspecified
Product TypeMicroplate
Target MoleculeGST
Total Volume (Metric) Well400 μL
Volume (Metric) Working100 μL
Array8 x 12
ColorWhite
FormatStandard
MaterialPolystyrene
No. of Wells96
SterilityNon-sterile
Surface TreatmentGlutathione
Volume (Metric) Well400 μL
Well ShapeFlat Bottom (F-well)
Unit SizeCase of 15

常見問答集 (常見問題)

What is the detection limit of proteins with Immobilizer Glutathione plates?

The background is extremely low when using the Immobilizer Glutathione plates. This results in a high signal-to-noise ratio and a low detection limit which in general means that the detection limit (OD 0.5) is 3 ng per well (100 µL).

How long are Immobilizer Glutathione plates stable?

The Immobilizer Glutathione plates are extremely stable over long periods of time, if stored at room temperature (20-25 degrees C). We guarantee 12 mths of shelf life in unopened foil package after the date of shipment.

With Immobilizer Glutathione plates, why should I use Tween-20 surfactant in the washing step and in the buffers containing the antibodies?

We recommend using 0.05% Tween-20 surfactant (as the non-ionic detergent) to improve the signal-to-noise ratio of the assay and thus decrease the background.

Should I optimize the concentration of the purified GST or GST-tagged protein to be used with Immobilizer Glutathione plates?

Yes. We recommend using a solution of the purified GST or GST-tagged protein diluted in Phosphate Buffered Saline (PBS), pH 7.2 in a concentration ranging from 0.01-1 µg/mL. During the coupling, non-ionic detergents like Tween-20 surfactant should not be present, as these will suppress the coupling.

Which type of molecules can I use with Immobilizer Glutathione plates?

The Immobilizer Glutathione plates are designed for optimal detection of glutathione-S-transferase (GST)-tagged proteins or purified GST.

View all Plates and Modules with Affinity Binding Surfaces FAQs