GeneAmp® Fast PCR Master Mix (2X), with protocol

Catalog number: 4362070

Applied Biosystems™  Related applications: PCR

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Description

The GeneAmp® Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti® 96-Well Thermal Cycler. Features of this master mix:

• Set up reactions quickly, using premixed reagents
• Detect a single copy of a gene in as little as 10 ng of DNA
• Get reproducible, specific, and sensitive results

Easy to use
Pre-mixed, hot-start chemistries enable you to set up your experiments quickly. And by generating greater than 90% "A" overhangs—it makes cloning easier too.

Detect a single copy in 10 ng of DNA
Use the GeneAmp® Fast PCR Master Mix to detect a single copy of a gene in 10 ng of human genomic DNA or as little as 10 copies of an HIV target.

Part of the Applied Biosystems® Fast PCR System
The Applied Biosystems® Fast PCR System, consisting of the premixed GeneAmp® Fast PCR Master Mix, specially designed microplates and tubes, and the Veriti® 96-Well Thermal Cycler (0.1 mL or 0.2 mL), considerably reduces PCR reaction times.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AmpliTaq® DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Medium
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Fast
Final Product Size(s): 2 kb or less
Product Size: 250 reactions
Exonuclease Activity: 5' - 3'

Contents & storage

The GeneAmp® Fast PCR Master Mix contains a hot start polymerase system that does not require an activation step and has been optimized to decrease the overall PCR amplification time. The Fast PCR Master Mix is provided at 2X concentration for easier preparation of PCR reactions requiring only the addition of primers and templates. The GeneAmp® Fast PCR Master Mix contains two tubes (1.25 mL each) sufficient for 125 20µL PCR reactions. The Fast PCR Master Mix can also be ordered without a protocol.

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