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Applied Biosystems™

7500 Fast Real-Time PCR System, tower, QST

Catalog number: 4365463
Applied Biosystems™

7500 Fast Real-Time PCR System, tower, QST

Catalog number: 4365463
Catalog Number
Unit Size
1 each
Price (USD)
Catalog NumberUnit SizePrice (USD)
43654631 eachContact Us ›
Product Overview
Citations & References
Additional Information
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. It is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM/SYBR Green I, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk, superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward

7500 Software v2.x
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.x upgrade. The 7500 Software v2.x incorporates your favorite StepOne Software features, such as multiple plate setup wizards, calculators for standard curve dilution and master mix recipes, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.x also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.

Gene Expression Study package accommodates large studies better than any other instrument software package
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results

21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.

• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow

Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, we provide preformulated, ready-to-use, quality-tested, TaqMan assays for use with the 7500 system. Now you can reduce your assay optimization efforts.

Upgrade to High-Speed Thermal Cycling
An optional upgrade to a the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.


Calibrated Dye
SYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
Data Acquisition
Collects data for all 5 filters for all wells regardless of plate setup
Reaction Speed
Fast, Standard
Dynamic Range
9 logs of linear dynamic range
External Computer
96-well plate
For Use With (Equipment)
7500 Fast System
High-throughput Compatibility
High-throughput Compatible, Multiplexing
Max. Power
950 W
Tungsten-halogen lamp, five-emission filters and CCD camera, Five-excitation filters
Passive Reference Dye
ROX (Separate Tube), ROX (Pre-mixed)
Can distinguish between 5000 and 10000 template copies with 99.7% confidence
Reaction Volume Range
10-30 µL
Sample Ramp Rate
Fast mode: ± 3.5°C/sec., Standard mode: ± 1.6°C/sec.
Down to 1 copy of RNase P gene from human genomic DNA
Software Compatibility
Primer Express Software, System Software
Temperature Accuracy
Within 0.5°C (35° to 95°C) of setpoint/display temp (3 min. after start)
Temperature Range (Metric)
4° to 100°C
Temperature Uniformity
Within 1.0°C (30 seconds after clock start)
Thermal Cycling System
Peltier-Based System
Manual loading of four-five 96-well plates per 8-hr day (real-time throughput)
34 cm (W) x 49 cm (H) x 45 cm (D) (13.39 x 19.29 x 17.72 in.)
Weight (English)
75 lb.
120 V