Applied Biosystems™
TaqMan™ MicroRNA Reverse Transcription Kit
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Description
The Applied Biosystems® TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for 200 reactions of optimal performance in TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Reverse Transcriptase: | MultiScribe™ |
|---|---|
| Downstream Application: | Real-Time Quantitative PCR (qPCR) |
| Reaction Format: | Separate Components |
| Sample Type (General): | RNA |
| Final Product: | cDNA |
| Final Product Size(s): | 200 bp or less |
| Optimal Reaction Temperature: | 42° C |
| Product Line: | TaqMan™ |
| Product Size: | 200 reactions |
| Shipping Condition: | Dry Ice |
Contents & storage
Contains 10X RT buffer, dNTP mix w/dTTp (100M total), RNase inhibitor (20U/µL), and MultiScribe™ RT enzyme (50U/µL) for 200 reactions. Store at -15 to -25°C.
