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Applied Biosystems™
TaqMan™ MicroRNA Reverse Transcription Kit
The Applied Biosystems TaqMan MicroRNA Reverse Transcription Kit provides the necessary components for 1000 reactions of optimal performance in TaqManRead more
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| Catalog Number | Sufficient For |
|---|---|
| 4366596 | 200 Reactions |
| 4366597 | 1000 Reactions |
Catalog number 4366596
Price (USD)/ Each
533.65
Online Exclusive
562.00Save 28.35 (5%)
Sufficient For:
200 Reactions
Price (USD)/ Each
533.65
Online Exclusive
562.00Save 28.35 (5%)
The Applied Biosystems TaqMan MicroRNA Reverse Transcription Kit provides the necessary components for 1000 reactions of optimal performance in TaqMan MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan MicroRNA Assay to convert miRNA to cDNA.
This is a highly specific kit that quantitates only mature miRNAs, not precursors and a sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is a fast, simple, and scalable two-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
Note: The amount of RT enzyme included in the kit is sufficient for single tubes but not for arrays.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration10X
Fidelity (vs. Taq)10 X
Final Product TypecDNA
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
No. of Reactions200 Reactions
Optimal Reaction Temperature42°C
Product LineTaqMan™
Product TypeMicroRNA Reverse Transcription Kit
Quantity200 reactions
Reaction FormatSeparate Components
Reverse TranscriptaseMultiScribe™
Sample TypeRNA
Shipping ConditionDry Ice
Size (Final Product)200 bp or less
Sufficient For200 Reactions
Detection MethodPrimer-probe
For Use With (Application)miRNA analysis
GC-Rich PCR PerformanceHigh
PCR Method2-step RT-qPCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
Contains 10X RT buffer, dNTP mix w/dTTp (100M total), RNase inhibitor (20U/μL), and MultiScribe™ RT enzyme (50U/μL) for 200 reactions. Store at -15 to -25°C.