The Applied Biosystems® TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for 1000 reactions of optimal performance in TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.
A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)
Real-Time Quantitative PCR (qPCR)
No. of Tests
200 Reactions
Optimal Reaction Temperature
42° C
Product Line
TaqMan™
Product Type
MicroRNA Reverse Transcription Kit
Reverse Transcriptase
MultiScribe™
Shipping Condition
Dry Ice
Sufficient For
200 Reactions
Concentration
10 X
Detection Method
Primer-Probe
Fidelity (vs. Taq)
10 X
Final Product Type
cDNA
GC-Rich PCR Performance
High
No. of Reactions
200 Reactions
PCR Method
RT-PCR
Reaction Format
Separate Components
Reaction Speed
Fast
Sample Type
RNA
Size (Final Product)
200 bp or less
Contents & Storage
Contains 10X RT buffer, dNTP mix w/dTTp (100M total), RNase inhibitor (20U/µL), and MultiScribe™ RT enzyme (50U/µL) for 200 reactions. Store at -15 to -25°C.
Figures
RT-PCR is step 2 in the workflow for TaqMan® MicroRNA Assays.
