Applied Biosystems™

      TaqMan™ MicroRNA Reverse Transcription Kit

      Catalog number:  4366596

       Related applications: miRNA & Non-Coding RNA Analysis

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      Description

      The Applied Biosystems® TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for 200 reactions of optimal performance in TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

      A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Reverse Transcriptase: MultiScribe™
      Downstream Application: Real-Time Quantitative PCR (qPCR)
      Reaction Format: Separate Components
      Sample Type (General): RNA
      Final Product: cDNA
      Final Product Size(s): 200 bp or less
      Optimal Reaction Temperature: 42° C
      Product Line: TaqMan™
      Product Size: 200 reactions
      Shipping Condition: Dry Ice

      Contents & storage

      Contains 10X RT buffer, dNTP mix w/dTTp (100M total), RNase inhibitor (20U/µL), and MultiScribe™ RT enzyme (50U/µL) for 200 reactions. Store at -15 to -25°C.

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