7500 Real-Time PCR System for Human Identification with Dell Notebook D520

Catalog number: 4366605

Applied Biosystems™  Related applications: Forensics | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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The Applied Biosystems® 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.

This instrument system provides the following:
• It is validated for use with the Quantifiler® Human Quantification and the Quantifiler® Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler® kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ® kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler® kit results in less than 2 hours.

Built for Performance
The 7500 system for Human Identification includes SDS v.1.2.3 software which is validated to run Quantifiler® Human DNA and Quantifiler® Y Human Male DNA quantification kits. SDS v1.2.3 is a powerful, versatile software that includes plate set-up wizards and automated analysis tools that make data processing easier. Its advanced data viewing capabilities make analysis less time consuming. The instrument is a powerful, five-color platform that is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, TAMRA™⁄CY3, ROX™⁄Texas Red, and CY5 dyes. Its specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets and its advanced multi-componenting algorithm minimizes spectral crosstalk making the instrument superior for multiplexing.

Complete Validated Solution
Applied Biosystems® offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® STR kits, instrumentation, software, and consumables. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD. The system has been internally validated by Applied Biosystems® following DAB⁄SWGDAM guidelines for use with Quantifiler® Human Quantification System and Quantifiler® Y Human Male DNA Quantification System making the 7500 system suitable for casework, paternity, and databasing laboratories.

When you partner with Applied Biosystems® you gain access to the extensive knowledge and experience of our forensic support team and training resources dedicated to Human Identification laboratories. Our HID University™ provides continuing education for DNA analysts, our support and Applications Scientists offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Format: 96-well plate
Optics: Tungsten-halogen lamp, 5 emission filters, CCD Camera, 5 excitation filters
Weight: 75 lbs
Precision: 99.7% confidence (5000 & 10000 copies)
Dimensions: Width: 34 cm (13.39 in), Height: 49 cm (19.29 in), Depth: 45 cm (17.72 in)
Throughput: 4 to 5-96-well plates per 8-hr day
Power Limit: 950 W
Sensitivity: 1 copy of RNase P gene (human genomic DNA)
Product Size: 1 system
Dynamic Range: 9 logs
Input Voltage: 120V
Calibrated Dye: SYBR® Green I, ROX™, JOE™, FAM™, VIC™, Cy5™, Cy3™, TAMRA™, NED™, Texas Red™
Reaction Speed: Standard
Sample Ramp Rate: ± 1.1°C⁄sec
External Computer: Dell Notebook D520
Peak Block Ramp Rate: 2.5°C⁄sec
Temperature Accuracy: 0.25°C (35°C to 95°C after 3 min)
Passive Reference Dye: ROX (Separate Tube), No ROX, ROX (Pre-mixed)
Reaction Volume Range: 20-100 µl
Software Compatibility: GeneMapper® ID v3.2, 7500 System Software, HID Real-Time PCR Analysis Software v1.1
Temperature Uniformity: 0.50°C (after 30 sec)
Thermal Cycling System: Peltier-Based System
For Use With (Equipment): 7500 System
Temperature Range (Metric): 4-100°C
High Throughput Compatibility: Multiplexing

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Manuals & protocols