Power SYBR™ Green PCR Master Mix
<i>Power</i> SYBR&trade; Green PCR Master Mix
実際の製品は異なる場合があります
<i>Power</i> SYBR&trade; Green PCR Master Mix
<i>Power</i> SYBR&trade; Green PCR Master Mix
<i>Power</i> SYBR&trade; Green PCR Master Mix
<i>Power</i> SYBR&trade; Green PCR Master Mix
Applied Biosystems™

Power SYBR™ Green PCR Master Mix

Get superior nucleic acid quantitation with Applied Biosystems™ Power SYBR™ Green Master Mix. Power SYBR™ Master Mix offers superior sensitivity詳細を見る
製品番号(カタログ番号)数量
43685771 x 1 mL
43676591 x 5 mL
43687062 x 5 mL
43687025 x 5 mL
436870810 x 5 mL
43676601 x 50 mL
製品番号(カタログ番号) 4368577
価格(JPY)
11,300
Each
お問い合わせください ›
数量:
1 x 1 mL
Get superior nucleic acid quantitation with Applied Biosystems™ Power SYBR™ Green Master Mix. Power SYBR™ Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

• Everything you need for SYBR™ Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR™ Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR™ Green reagent-based real-time PCR
• The optimized formulation contains highly purified AmpliTaq Gold™ DNA Polymerase, LD to offer greater sensitivity than classic SYBR™ Green PCR master mix
Power SYBR™ Green PCR Master Mix contains a blend of dTTP/dUTP that is compatible with AmpErase™ UNG, to minimize carryover contamination
• Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations to minimize well-to-well variability that result from a variety of causes, such as pipetting error and sample evaporation
Power SYBR™ Green reagent-based PCR chemistry easily replaces the existing SYBR™ Green PCR Master Mix in Applied Biosystems™ protocols using the same setup and thermal cycling conditions

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Power SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
フォーマットTube
反応数反応40回分
受動的参照色素ROX(プレミックス)
ポリメラーゼAmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
製品ラインSYBR
製品タイプReal Time PCR SYBR Master Mix
純度または品質グレードLD(低DNA)
数量1 x 1 mL
サンプルタイプDNA (Genomic), cDNA
出荷条件Wet Ice
対応可能対象40 Reactions of 50 μL
容量1 x 1 mL
濃度2X
検出法SYBR
使用対象(アプリケーション)遺伝子発現解析
GC-Rich PCR Performance
標識または色素SYBR Green
PCR法qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
The 2x mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1 and optimized buffer components. Contains 1 X 1 mL vial, sufficient for 40 reactions at 50 μL. Store at -20°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

よくあるご質問(FAQ)

Power SYBR Green PCR Master MixはSYBR Green PCR Master Mixの代用品として利用できますか?

Power SYBR Green PCR Master Mix (P/N 4368577/ 4367659/ 4368706/ 4368702/ 4368708/ 4367660)は、従来品のSYBR Green PCR Master Mix (P/N 4344463/ 4309155/ 4364344/ 4364346/ 4312704/ 4334973)を、改良した新しい試薬です。 Power SYBR PCR Master Mixには、従来品のSYBR Green PCR Master Mixと比較し、より精製純度の高い AmpliTaq 酵素を採用しており、Bufferの組成においても、さらなる最適化が施されています。なおPower SYBR Green PCR Master Mixに変更した場合の、検証方法の詳細情報は、Power SYBR Green PCR Master Mix Protocol (P/N 4367218)を参照してください.

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.